This product is Cohn fraction V Albumin prepared by a modified method of Cohn cold alcohol precipitation. It is not tested for protease content. It is suitable for general cell culture applications, so it is expected to be suitable for organoid culture. Please refer to the Albumin Application Selection Guide at the link below for additional details and navigate to the DOCUMENTATION section of the Product Detail Page to 'View Sample CoA', or to access a lot-specific Certificate under ‘Certificate of Analysis’:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/111/538/albumin-application-selection-guide.pdf
A9418
Seroalbúmina bovina
lyophilized powder, BioReagent, suitable for cell culture
Sinónimos:
Albúmina bovina, Albúmina sérica bovina, BSA
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$526.000
En stockDetalles
Seleccione un Tamaño
About This Item
$526.000
En stockDetalles
Productos recomendados
origen biológico
bovine
Nivel de calidad
Línea del producto
BioReagent
Ensayo
≥96% (agarose gel electrophoresis)
Formulario
lyophilized powder
mol peso
~66 kDa
origen
USA origin
técnicas
cell culture | mammalian: suitable
impurezas
BT and VSV virus, tested
pérdida
≤5%
pH
5.0-5.6
solubilidad
H2O: 40 mg/mL
actividad extraña
BT virus, none detected
VSV virus, none detected
Condiciones de envío
ambient
temp. de almacenamiento
2-8°C
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Descripción general
Aplicación
Acciones bioquímicas o fisiológicas
Nota de preparación
producto comparable
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Equipo de protección personal
Eyeshields, Gloves, type N95 (US)
Elija entre una de las versiones más recientes:
Certificados de análisis (COA)
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Contenido relacionado
Learn about bovine serum albumin (BSA) structure, molecular weight, function, solubility, how BSA is made, purification methods, applications and purchasing.
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Is BSAS A9418, Protease-free and suitable for Organoid Culture (Growth Factor preparations)? This Cat# doesn't say Fraction-V? Is it protease free or not?
1 answer-
Helpful?
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Hello, Can you send me the amino acid sequence and the Uniprot or PDB file?
1 answer-
Historically, the molecular weight of BSA has been cited as 66,1201 or 66,2672. However, it was revised in 1990 to 66,4303. All three values are based on amino acid sequence information available at the time of publication. The complete sequence can be found in the UniProt
https://www.uniprot.org/uniprotkb/P02769/entryPlease see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/250/584/a9418pis.pdfHelpful?
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I make a mistake to put this BSA powder (A9647-100G) in the room temperature for 39 hours. Can it still work?
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The long term storage recommendation for this product is refrigerated at 2-8 °C, but it is shipped at ambient temperature. Refrigerated products that ship at ambient temperature are stable for up to 2 weeks to allow for transit time. Items left at room temperature for short periods will not be adversely effected.
Helpful?
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Would it be safe to use product A9418 if it was accidentally stored at -20°C for approximately 24 hours, even though it is intended to be stored at 2-8°C?
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The performance of the item after storing it at -20°C cannot be guaranteed, and testing its functionality is recommended.
Helpful?
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Can BSA ever contain alpha1 anti-trypsin as a contaminant?
1 answer-
The available Bovine Serum Albumin products are not tested for the presence of alpha-1 antitrypsin (AAT). AAT is typically found in Bovine Serum, however, it is unknown if the isolation and purification methods employed in the production of BSA would degrade this protein. A brief search of the literature shows AAT has a molecular weight range of 45 - 52 kDa. It is irreversibly degraded at pH levels below 5. A further review of the literature on potential removal or purification would be recommended.
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