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PROTDT

Millipore

ProteoPrep® Detergent Sample Kit

Sinonimo/i:

protein solubilization detergent

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1 KIT
CHF 490.00

CHF 490.00

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1 KIT
CHF 490.00

About This Item

Codice UNSPSC:
12352200
NACRES:
NA.32

CHF 490.00

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Durata

2 yr

Temperatura di conservazione

15-25°C

Categorie correlate

Descrizione generale

The ProteoPrep® Detergent Sample Kit provides ten detergents, non-ionic and zwitterionic, for protein solublization. The variety of innovative detergents enables optimization of extraction methods, which may vary with the protein of interest and cell type. Zwitterionic detergents uniquely offer some intermediate class properties that are superior to other detergent types. Each detergent is supplied in a convenient package size, ideal for method development and experimentation.

Applicazioni

ProteoPrep® Detergent Sample Kit has been used as a component of sample buffer for hepatoblastoma (HB) HepG2 cells.[1]

Note legali

ProteoPrep is a registered trademark of Merck KGaA, Darmstadt, Germany

Solo come componenti del kit

N° Catalogo
Descrizione
  • Brij® C10 1 g

I componenti del kit sono disponibili anche separatamente

N° Catalogo
Descrizione
SDS
  • A1346ASB-14 1 gSDS
  • C0856C7BzO 1 gSDS
  • P2393Polyoxyethylene (10) tridecyl ether, mixture of C11 to C14 iso-alkyl ethers with C13 iso-alkyl predominating 1 gSDS
  • C9426CHAPS hydrate, BioReagent, suitable for electrophoresis, ≥98% (HPLC) 1 gSDS
  • D4641n-Dodecyl β-D-maltoside, ≥98% (GC) 1 gSDS
  • O8001Octyl β-D-glucopyranoside, ≥98% (GC) 1 gSDS
  • O6004Octyl β-D-1-thioglucopyranoside, ≥98.0% (GC) 1 gSDS
  • D42663-(Decyldimethylammonio)­propane­sulfonate inner salt, zwitterionic detergent 1 gSDS
  • T8532Triton™ X-100, suitable for electrophoresis 1 mLSDS
Vedi tutto (9)

Avvertenze

Danger

Indicazioni di pericolo

H302,H315,H318,H360D,H410

Classi di pericolo

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Repr. 1B - Skin Irrit. 2

Codice della classe di stoccaggio

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

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Certificati d'analisi (COA)

Lot/Batch Number
SLCD5127
SLBX7013
SLBT0335
SLBT0334
SLBQ0288V

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I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.

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NOX1 supports the metabolic remodeling of HepG2 cells
Bertram K, et al.
Testing, 10(3), e0122002-e0122002 (2015)
Katharina Bertram et al.
PloS one, 10(3), e0122002-e0122002 (2015-03-26)
NADPH oxidases are important sources of reactive oxygen species (ROS) which act as signaling molecules in the regulation of protein expression, cell proliferation, differentiation, migration and cell death. The NOX1 subunit is over-expressed in several cancers and NOX1 derived ROS

Questions

  1. How much of each surfactant and in what state (solid or solution, if solution what concentration) are the surfactants provided in this kit?

    1 answer

    1. As listed in the product data-sheet:

      Components
      CHAPS (Catalog Number C9426) 1 g - powder form
      C7BzO (Catalog Number C0856) 1 g - powder form
      ASB-14 (Catalog Number A1346) 1 g - powder form
      3-(Decyldimethylammonio) propanesulfonate inner salt (Catalog Number D4266) 1 g - powder form
      n-Dodecyl b-D-maltoside (Catalog Number D4641) 1 g - powder form
      Octyl b-D-glucopyranoside (Catalog Number O8001) 1 g - powder form
      Octyl b-D-1-thioglucopyranoside (Catalog Number O6004) 1 g - powder form
      Polyoxyethylene 10 tridecyl ether mixture of C11 to C14 iso-alkyl ethers with C13 iso-alkyl predominating (Catalog Number P2393) 1 g - liquid form, neat
      Brij C10 (Catalog Number P5759) 1 g - 'solid' form, melting point 32 - 34 °C, density in liquid form 0.977 g/mL
      TRITON X-100 (Catalog Number T8532) 1 ml - liquid, neat

      For more information please access the product data sheet: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/102/026/protdtbul.pdf

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