M0446
Minimum Essential Medium Eagle, with Earl's salts
StableCell™, with Earle′s salts, stable glutamine, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
Sinonimo/i:
Terreno essenziale minimo di Eagle, MEM
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About This Item
Codice UNSPSC:
12352207
NACRES:
NA.75
Prodotti consigliati
Nome del prodotto
Minimum Essential Medium Eagle, With Earle′s salts, stable glutamine, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
Livello qualitativo
Sterilità
sterile-filtered
Stato
liquid
tecniche
cell culture | mammalian: suitable
Impurezze
endotoxin, tested
Componenti
sodium pyruvate: no
HEPES: no
Hanks’ salts (2% CO2): no
NaHCO3: yes
Earle’s salts (5% CO2): no
stable glutamine: yes
phenol red: yes
L-glutamine: no
glucose: yes
Condizioni di spedizione
ambient
Temperatura di conservazione
2-8°C
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Descrizione generale
Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media. Early attempts to cultivate normal mammalian fibroblasts and certain subtypes of HeLa cells revealed they had specific nutritional requirements that could not be met by Eagle′s Basal Medium (BME). Subsequent studies using these and other cells in culture indicated additions to BME could be made to aid growth of a wider variety of fastidious cells.
MEM, which incorporates these modifications, includes higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. MEM has been used for cultivation of a wide variety of cells grown in monolayers. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks′ or Earle′s salts has broadened the usefulness of this medium.
MEM, which incorporates these modifications, includes higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. MEM has been used for cultivation of a wide variety of cells grown in monolayers. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks′ or Earle′s salts has broadened the usefulness of this medium.
Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.
Applicazioni
Minimum Essential Medium Eagle has been used in murine fibroblast culture for in vitro cytocompatibility testing.
Altre note
This product lacks L-Ala; L-Asn; L-Glu; Gly; L-Pro; L-Ser and L-Gln. It is supplemented with L-Ala-L-Gln dipeptide. This provides a more stable form of glutamine for cell culture. Free amino acid L-glutamine is known to be unstable in cell culture.
Note legali
StableCell is a trademark of Sigma-Aldrich Co. LLC
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Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves
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