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M0268

Terreno essenziale minimo di Eagle

With Earle′s salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Sinonimo/i:

MEM

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10 L
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CHF 50.40
10 x 1 L
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CHF 69.80
50 L
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CHF 190.00

Informazioni su questo articolo

NACRES:
NA.75
UNSPSC Code:
12352207

CHF 50.40


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form

powder

technique(s)

cell culture | mammalian: suitable

components

HEPES: no
phenol red: yes
Earle’s salts (5% CO2): yes
NaHCO3: no
L-glutamine: yes
sodium pyruvate: no

shipped in

ambient

storage temp.

2-8°C

General description

Minimum Essential Medium Eagle (MEM) is one of the most widely used synthetic cell culture media. MEM has been used for culturing several cells grown in monolayers. It is commonly used to grow attached cell lines, such as fibroblasts, in the presence of fetal bovine sera (FBS), calf, or horse sera.

Application

Minimum Essential Medium Eagle has been used:
  • in the isolation and culture of primary hepatocytes
  • to culture the human endometrial carcinoma cells Ishikawa (ATTC) and L-Wnt-3a cells (ATCC) that secrete Wnt family member 3a (Wnt-3a) ligand
  • to culture the AN3CA (endometrial cancer cell line) cells, and HEC1-B human (endometrial adenocarcinoma cell line) cells

Features and Benefits

  • Minimum Essential Medium Eagle (MEM) contains higher concentrations of amino acids to mimic the protein composition of cultured mammalian cells.
  • The formulation has been altered by optional elimination of calcium to enable the growth of cells in suspension culture.

Preparation Note

Formulated to contain 9.5 grams of powder per liter of medium.
Supplement with 2.2 g/L sodium bicarbonate.

Other Notes

Essentially the same as the original formula, except it is provided as a powder.

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Questo articolo
M2279M5650M0644
form

powder

form

liquid

form

liquid

form

powder

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

components

HEPES: no
phenol red: yes
Earle’s salts (5% CO2): yes
NaHCO3: no
L-glutamine: yes
sodium pyruvate: no

components

phenol red: yes
sodium pyruvate: no
L-glutamine: no
HEPES: no
NaHCO3: yes
Earle’s salts (5% CO2): yes

components

phenol red: yes
NaHCO3: yes
L-glutamine: no
sodium pyruvate: no
HEPES: no
Earle’s salts (5% CO2): yes

components

NaHCO3: no
L-glutamine: yes
phenol red: yes
HEPES: no
sodium pyruvate: yes

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

classic media, classical media, FBS

classic media, classical media, MEM, classic media, classical media, FBS

classic media, classical media, MEM, classic media, classical media, FBS

classic media, classical media, FBS, hybrid cells


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wgk

WGK 2

Classe di stoccaggio

13 - Non Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable



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Questions

1–5 of 5 Questions  
  1. Does Product M0268, Minimum Essential Medium Eagle, contain phenol red?

    1 answer
    1. Yes, this product contains phenol red.  Phenol red is included in most medium as a visual pH indicator.  The medium should be changed when the color turns yellow (acidic) or purple (basic).

      Helpful?

  2. What is the formulation of Product M0268, Minimum Essential Medium Eagle?

    1 answer
    1. The formulation for this product can be found on the product datasheet.

      Helpful?

  3. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  4. Does Product M0268, Minimum Essential Medium Eagle, have an expiration date?

    1 answer
    1. Yes, this product has an expiration date of 36 months from the date of manufacture. The expiration date can be found on the Certificate of Analysis.

      Helpful?

  5. Does Product M0268, Minimum Essential Medium Eagle, contain sodium bicarbonate?

    1 answer
    1. No, this product does not contain sodium bicarbonate. It is recommended to add 2.2 g sodium bicarbonate per Liter of medium. Use either Product No. S5761 (powder) or S8761 (7.5% solution).

      Helpful?

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