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H7140

Sigma-Aldrich

Hybridization Solution

for Northern and Southern blotting, liquid (2x concentrate)

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About This Item

Codice UNSPSC:
12161700
NACRES:
NA.25

Livello qualitativo

100

Forma fisica

liquid (2x concentrate)

Attività estranea

DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected

Temperatura di conservazione

−20°C

Categorie correlate

Descrizione generale

Sigma′s Hybridization Solution is a 2x ready-to-use hybridization buffer for Northern and Southern blot protocols.

Applicazioni

Hybridization Solution has been used to hybridise the nitrocellulose membrane. It has also been used in DNA hybridization.
Suitable for use as a hybridization buffer in Northern and Southern blot protocols.

Componenti

Sigma′s Hybridization Solution contains 200 μg/mL DNA in 10x SSC and 2X Denhardt′s solutions:
200 μg/mL DNA
2mg Bovine Serum Albumin (BSA)
2mg Ficoll
2mg Polyvinylpyrrolidone (PVP)
0.15 M sodium citrate
1.5 M NaCl

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Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Protocolli

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

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