If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf
T9285
Tampone Tris-EDTA
100X concentrate for Northern and Southern blotting
Sinonimo/i:
Tampone di lavaggio Tris-EDTA, Tampone TE
Scegli un formato
| A voi/SKU | Disponibilità | Prezzo |
|---|---|---|
100 mL | Per conoscere la disponibilità, visualizza il carrello | CHF 79.10 CHF 67.23 |
Informazioni su questo articolo
CHF 67.23
Prezzo di listinoCHF 79.10Risparmia il 15%grade
Molecular Biology
Quality Segment
sterility
0.2 μm filtered
form
liquid
pH
7.8-8.2 (1 × in water)
application(s)
sample preparation
foreign activity
DNase, RNase, none detected
storage temp.
room temp
SMILES string
NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
InChI
1S/C10H16N2O8.C4H11NO3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2
InChI key
VLEIUWBSEKKKFX-UHFFFAOYSA-N
General description
Application
Preparation Note
Other Notes
1 of 1
Questo articolo | |||
|---|---|---|---|
| grade Molecular Biology | grade Molecular Biology | grade Molecular Biology | grade Molecular Biology |
| Quality Level 200 | Quality Level 200 | Quality Level 100 | Quality Level 100 |
| form liquid | form liquid | form liquid | form solution |
| application(s) sample preparation | application(s) sample preparation | application(s) - | application(s) - |
| pH 7.8-8.2 (1 × in water) | pH 8.0±0.2 (25 °C) | pH 7.4±0.2 (25 °C) | pH 8.6-9.0 |
| storage temp. room temp | storage temp. - | storage temp. - | storage temp. room temp |
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Classe di stoccaggio
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
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How can I determine the shelf life / expiration / retest date of this product?
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What is the molarity of the 100X Tris EDTA solution?
1 answer-
At the 100X concentration this solution contains 1M Tris-HCl and 0.1M EDTA. When diluted to 1X (1:100), the solution is contains 10mM Tris-HCL and 1mM EDTA.
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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Can this Tris-EDTA buffer solution be used in an IHC protocol for antigen retrieval?
1 answer-
This product has not been tested specifically for antigen retrieval. The researcher would have to determine suitability. Please see the link below to product E1161. This product is a prepared 10X solution formulated specifically for retrieval and may be better suited the specified application:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/374/855/e1161pis.pdfHelpful?
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What is the pH and color of Product T9285, Tris-EDTA buffer solution?
1 answer-
The pH and color information is available on the specification sheet as well as lot specific Certificate of Analysis.
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What does 'for molecular biology' mean?
1 answer-
The phrase `for molecular biology' means that all lots of this buffer have been tested for DNase and RNase activity, and that no activity was detected.
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What is the shelf life of Product T9285, Tris-EDTA buffer solution?
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This buffer solution has a recommended retest date of three years. This information is available on the Specification Sheet, and on the lot-specific Certificates of Analysis.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What are the final concentrations of Tris and EDTA when Product T9285 is diluted to 1:100 to make it 1X?
1 answer-
When diluted 1:100, the solution is 10mM TRIS with 1mM EDTA.
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