Passa al contenuto
Merck
Tutte le immagini(1)

Documenti

D9156

Sigma-Aldrich

Deoxyribonucleic acid, single stranded from salmon testes

For hybridization

Sinonimo/i:

single-stranded template DNA

Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
Tutte le immagini(1)

About This Item

Numero CAS:
68938-01-2
Numero MDL:
MFCD00130921
Codice UNSPSC:
41106310
eCl@ss:
32160414
NACRES:
NA.52

Origine biologica

fish testis (salmon)

Livello qualitativo

200

Grado

for molecular biology

Saggio

9-11 mg/mL (DNA concentration)

Forma fisica

solution

Concentrazione

10 mg/mL in H2O

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

Cerchi prodotti simili? Visita Guida al confronto tra prodotti

Categorie correlate

Descrizione generale

A ready-to-use solution of high quality single-stranded template DNA isolated from the testes of salmon. The solution is supplied at a concentration of 9 - 12 mg/ml .

Applicazioni

Deoxyribonucleic acid, single stranded from salmon testes is suitable for use as a blocking agent in Southern hybridizations. It was used for in situ hybridization histochemistry of bone marrow biopsy samples and human blood monocyte subpopulations.
Many factors contribute to the signal-to-noise ratio in nucleic acid hybridizations. These factors include the presence of solvent (formamide), hybridization temperature, length of hybridization, volume of hybridization solution, degree and method of agitation, use of blocking reagents, concentration and specific activity of the probe, use of molecular agents to increase the rate of nucleic acid reassociation, and the degree of stringency used during the washing of the membrane.

In order to decrease any non-specific hybridization of the probe to a substrate, blocking agents must be used. Generally, a combination of blocking reagent, detergent, and denatured, fragmented DNA is used to accomplish this. Sigma offers sonicated, denatured DNA from a variety of species for use as a blocking agent in Northern and Southern blotting and other nucleic acid hybridization techniques.

Nota sulla preparazione

This DNA is ethanol precipitated and sonicated to produce single-stranded fragments which comigrate with the 587 and 831 base pair marker fragments.

Altre note

DNA in solution will reanneal on standing at room temperature so it is recommended to boil the solution for 10 minutes and then cool on ice for at least 5 minutes prior to use.

Prodotti correlati

N° Catalogo
Descrizione
Determinazione del prezzo

D4522

Deoxyribonucleic acid from calf thymus, Type XV, Activated, lyophilized powder

D3287

Deoxyribonucleic acid, single stranded from human placenta

D4889

Deoxyribonucleic acid sodium salt from Escherichia coli strain B, Genomic, unsheared

D7290

Deoxyribonucleic acid from herring sperm, single-stranded for hybridization

D7656

Deoxyribonucleic acid, single stranded from salmon testes, For hybridization

D3035

Deoxyribonucleic acid from human placenta, buffered aqueous solution, sexed, female

D3160

Deoxyribonucleic acid from human placenta, buffered aqueous solution, sexed, male

D8661

Deoxyribonucleic acid solution from calf thymus, For hybridization

S8140

SSPE Buffer, for Northern and Southern blotting, powder blend

N6019

Neutralizing Buffer, for Northern and Southern blotting, powder

N1531

Denaturation Solution, for Northern and Southern blotting

B6429

Casein Blocking Buffer 10x, for Northern and Southern blotting, solution

S2015

SSPE Buffer 20× Concentrate, for Northern and Southern blotting, solution

M5755

MOPS-EDTA-Sodium Acetate (MESA), powder for RNA electrophoresis buffers

S0902

SSC Buffer, for Northern and Southern blotting, powder blend

H7033

Tampone per ibridazione PerfectHyb™ Plus, for Northern and Southern blotting, solution

S8015

SSC Buffer, for Northern and Southern blotting, powder blend

S6639

SSC Buffer 20× Concentrate, for Northern and Southern blotting, solution

P1415

Prehybridization Solution, for Northern and Southern blotting, liquid (2x concentrate)

C7594

Casein Blocking Buffer, for Northern and Southern blotting, powder blend

15356

Blotting-Nylon 66 membranes, type B, positive, for Northern and Southern blotting

31149

Deoxyribonucleic acid, low molecular weight from salmon sperm

GE27-4565-01

Salmon Testes Sonicated DNA, Cytiva 27-4565-01, pack of 1 ea

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

Possiedi già questo prodotto?

I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.

Visita l’Archivio dei documenti

H W Ziegler-Heitbrock et al.
Blood, 79(2), 503-511 (1992-01-15)
Cytokine expression was analyzed in CD14++ regular monocytes and in the novel subset of CD14+/CD16+ small monocytes. Biologic activity for tumor necrosis factor (TNF), interleukin-1 (IL-1), and IL-6 in the supernatant of elutriator-enriched, cell sorter-purified small monocytes was about 10-fold
J W Said et al.
Blood, 90(11), 4278-4282 (1997-12-31)
We have recently demonstrated the presence of Kaposi's sarcoma-associated herpesvirus (KSHV) in cultured bone marrow (BM) stromal dendritic cells from all patients with myeloma studied. To show that these findings were not an artifact of tissue culture, we performed in
Marion Herbette et al.
PLoS genetics, 17(7), e1009662-e1009662 (2021-07-07)
Segregation Distorter (SD) is a male meiotic drive system in Drosophila melanogaster. Males heterozygous for a selfish SD chromosome rarely transmit the homologous SD+ chromosome. It is well established that distortion results from an interaction between Sd, the primary distorting
Manolis Kellis et al.
Proceedings of the National Academy of Sciences of the United States of America, 111(17), 6131-6138 (2014-04-23)
With the completion of the human genome sequence, attention turned to identifying and annotating its functional DNA elements. As a complement to genetic and comparative genomics approaches, the Encyclopedia of DNA Elements Project was launched to contribute maps of RNA
Zachary D Smith et al.
Nature, 511(7511), 611-615 (2014-08-01)
In mammals, cytosine methylation is predominantly restricted to CpG dinucleotides and stably distributed across the genome, with local, cell-type-specific regulation directed by DNA binding factors. This comparatively static landscape is in marked contrast with the events of fertilization, during which
Visualizza tutti i documenti correlati

Articoli

Yeast Transformation Introduction

Transformation introduces exogenous DNA into cells, a fundamental genetic modification process demonstrated in Streptococcus pneumoniae.

Protocolli

Yeast Transformation Protocols

Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells.

Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

Contatta l'Assistenza Tecnica.