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S0902

SSC Buffer

Name: SSC Buffer
Brand: SIGMA

for Northern and Southern blotting, powder blend

Sinonimo/i:

Saline-sodium citrate buffer

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Informazioni su questo articolo

NACRES:

NA.25

UNSPSC Code:

41105319

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Proprietà

Quality Segment

200

form

powder blend

pH

6.8-7.2 (20-25 °C, 263 mg/L in water)

solubility

water: 263 mg/mL, clear, colorless

foreign activity

DNAse, none detected, Endonuclease, none detected, Exonuclease, none detected, NICKase, none detected, RNAse, none detected

storage temp.

room temp

Descrizione

General description

SSC Buffer 20x Powder is a standard reagent in Southern and Northern blotting procedures. This concentrated powder should be reconstituted in molecular biology grade water to the indicated final volume before use (final solution is a 20x). The 20x concentrate can be used straight or diluted to make SSC working solutions with concentrations as low as 0.5x.

Application

Saline-sodium citrate (SSC) Buffer has been used as a washing buffer in microarray technique. It has also been used as a constituent of fluorescence in situ hybridization (FISH) buffer.

Suitable for:

Preparing nucleic acids for hybridization
Hybridization buffer for Northern and Southern blots
Washing buffer for Northern and Southern blots

Features and Benefits

Multi-purpose use in hybridizations
Easily reconstituted and diluted to any concentration needed

Preparation Note

Final SSC Buffer 20x Concentrate contains 0.3 M sodium citrate in 3M NaCl.

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Questo articolo	85639	S8140	93017
Sigma-Aldrich S0902 SSC Buffer	Sigma-Aldrich 85639 SSC Buffer	Sigma-Aldrich S8140 SSPE Buffer	Sigma-Aldrich 93017 SSC Buffer 20x
description for Northern and Southern blotting, powder blend	description for Northern and Southern blotting, powder blend	description for Northern and Southern blotting, powder blend	description for Northern and Southern blotting, solution
Quality Level 200	Quality Level 100	Quality Level 200	Quality Level 200
form powder blend	form powder blend	form powder blend	form solution
pH 6.8-7.2 (20-25 °C, 263 mg/L in water)	pH -	pH 7.2-7.6 (20 × in water, high purity, ambient)	pH 7.0±0.2
storage temp. room temp	storage temp. room temp	storage temp. room temp	storage temp. room temp
solubility water: 263 mg/mL, clear, colorless	solubility H₂O: 0.263 g/mL, clear, colorless	solubility water, high purity: 20 ×, clear, colorless	solubility -
foreign activity RNAse, none detected, DNAse, none detected, Endonuclease, none detected, Exonuclease, none detected, NICKase, none detected	foreign activity -	foreign activity RNAse, none detected, Endonuclease, none detected, NICKase, none detected, Exonuclease, none detected, DNAse, none detected	foreign activity -

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Classe di stoccaggio

13 - Non Combustible Solids

Che succede?

WGK 1

Punto di infiammabilità (°F)

Not applicable

Punto di infiammabilità (°C)

Not applicable

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