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P1415

Sigma-Aldrich

Prehybridization Solution

for Northern and Southern blotting, liquid (2x concentrate)

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About This Item

Codice UNSPSC:
12161700
NACRES:
NA.25

Livello qualitativo

Forma fisica

liquid (2x concentrate)

Attività estranea

DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected

Temperatura di conservazione

−20°C

Descrizione generale

Sigma′s Prehybridization Solution is a 2x ready-to-use buffer for Northern and Southern blot protocols.

Applicazioni

Suitable for use as a pre-hybridization buffer in Northern and Southern blot protocols.

Componenti

Sigma′s 2X Prehybridization Solution contains 200 μg/mL DNA in 10x SSC and 2X Denhardt′s solutions:
  • 200 μg/mL DNA
  • 2mg Bovine Serum Albumin (BSA)
  • 2mg Ficoll
  • 2mg Polyvinylpyrrolidone (PVP)
  • 0.15 M sodium citrate
  • 1.5M NaCl

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

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Protocolli

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

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