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B6185

Bionic Buffer, 10X Concentrate

for Northern and Southern blotting, solution

Sinonimo/i:

Sodium Borate Electrophoresis Buffer

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Informazioni su questo articolo

NACRES:
NA.25
UNSPSC Code:
41105319
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grade

Molecular Biology

Quality Segment

description

Mode of use Voltage of 150 V is recommended for small electrophoresis chambers while a mid to large apparatus may be run at 200-250 V. User should test their chamber to determine optimum voltage.

form

solution

concentration

10 ×

impurities

DNase, RNase and nickase, none detected

pH

8.0-8.5 (20-25 °C)

storage temp.

room temp

General description

Bionic Buffer is a unique alternative to traditional TBE (TRIS borate-EDTA) and TAE (TRIS acetate-EDTA) electrophoresis buffers. Bionic Buffer allows for higher voltage runs, extremely rapid running times, and high-resolution band separation.

Application

Suitable for:
  • Use as running buffer for nucleic acid electrophoresis, which may be part of Northern and Southern blot hybridization procedures
  • Use with TBA and TAE gels
  • Preparation of agarose gels

Features and Benefits

  • Faster run times
  • Sharper bands
  • Easy to dilute and use
  • Flexible use with a variety of gel types

Packaging

The 4 L and 10 L sizes are supplied in dispensers with a spigot.

Legal Information

Bionic is a trademark of Sigma-Aldrich Co. LLC

Disclaimer

Test electrophoresis chambers to determine highest voltage. Voltages beyond the recommended range for your apparatus may result in blown fuses or damage to your apparatus.

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Questo articolo
B6429B493093290
grade

Molecular Biology

grade

Molecular Biology

grade

Molecular Biology

grade

Molecular Biology

concentration

10 ×

concentration

-

concentration

-

concentration

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100

form

solution

form

solution

form

liquid

form

solution

pH

8.0-8.5 (20-25 °C)

pH

-

pH

-

pH

8.6-9.0

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp


pictograms

Health hazardExclamation mark

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Repr. 1B

Classe di stoccaggio

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter



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