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Documenti fondamentali

F2637

Sigma-Aldrich

Polisaccarosio 400

BioXtra, for molecular biology, lyophilized powder

Sinonimo/i:

Polysucrose 400, Ficoll 400

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About This Item

Numero CAS:
26873-85-8
Numero MDL:
MFCD00081599
Codice UNSPSC:
12352200
NACRES:
NA.52

Origine biologica

non-animal source

Livello qualitativo

200

Grado

Molecular Biology
for molecular biology

Nome Commerciale

BioXtra

Stato

lyophilized powder

Attività estranea

DNase (Exonuclease), NICKase (Endonuclease), RNase and Protease, none detected

Temperatura di conservazione

room temp

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Descrizione generale

Ficoll® 400 is a non-ionic synthetic polymer of sucrose used for cell separation and organ isolation.

Applicazioni

È usato nella separazione di cellule e nell′isolamento di organelli.
Suitable for
  • hybridization experiments to reduce non-specific binding of labeled probes to the membrane
  • preparation of DNA loading buffer (15% Ficoll®, 0.25% bromophenol blue and 0.25% xylene cyanol FF
  • separation of cells with similar density but different size (PBMC from human peripheral blood, bone marrow and umbilical cord blood)
  • producing density gradients to separate subcellular components
  • vitrification of cell and tissue samples.
  • to compare the effect of different perfusates on Hypoxic pulmonary vasoconstriction (HPV).

Caratteristiche e vantaggi

  • free from DNases, RNases, phosphatases and proteases
  • preserves functional and morphological integrity of the separated cells

Altre note

Un polimero non ionico sintetico del saccarosio.

Note legali

Ficoll is a registered trademark of Cytiva

Prodotti correlati

N° Catalogo
Descrizione
Determinazione del prezzo

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

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Certificati d'analisi (COA)

Lot/Batch Number
0000353416
0000353417
0000317775
0000317776
0000317778

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Hae Young Yoo et al.
Pulmonary circulation, 3(2), 396-405 (2013-09-10)
Hypoxic pulmonary vasoconstriction (HPV) is a compensatory physiological mechanism in the lung that optimizes the matching of ventilation to perfusion and thereby maximizes gas exchange. Historically, HPV has been primarily studied in isolated perfused/ventilated lungs; however, the results of these
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