C8571

Cathepsin B from human liver

buffered aqueous solution, ≥1,500 units/mg protein (E1%/280)

• Sinonimo/i: Cathepsin B₁
• Numero CAS: 9047-22-7
• Classificazione EC (Enzyme Commission): 3.4.22.1 (BRENDA, IUBMB)
• Numero MDL: MFCD00130748
• Codice UNSPSC: 12352200
• NACRES: NA.32

Proprietà

• Origine biologica: human liver
• Livello qualitativo: 200
• Stato: buffered aqueous solution
• Attività specifica: ≥1,500 units/mg protein (E1%/280)
• N° accesso UniProt: P07858
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −70°C
• Informazioni sul gene: human ... CTSB(1508)

Descrizione

Descrizione generale

Cathepsin B gene is mapped to human chromosome 8p23.1.[1] It is a lysosomal cysteine with 252 amino acids distributed in two chains and is homologous to rat liver sequence.[2] It has disc shaped structure with two domains namely the left-hand ′L′ domain at the amino terminal and right hand ′R′ domain at the carboxyl-terminal.[3]

Applicazioni

Cathepsin B from human liver has been used:

• as a positive control in the screening Cathepsin B inhibitor assay[4]
• in wall extension (expansin activity) assay[5]
• in microinjection human foreskin fibroblasts to test its effect on apoptosis induction[6]

Azioni biochim/fisiol

Cathepsin B from human liver is highly expressed in the esophageal adenocarcinoma (EAC).[1] It reduces hepatic inflammation and fibrogenesis and may serve as a potential candidate for treating liver diseases.[7]

Cathepsin B has been found to cleave procaspase 1 and procaspase 11 and to induce apoptosis in digitonin-permeabilized cells. Translocation of cathepsin B from the cytoplasm to the nucleus contributes to bile salt induced apoptosis of rat hepatocytes. Levels of cathepsin B in PC12 cells significantly decrease 12 to 24 hours after apoptosis is induced.

Found to cleave procaspase 1 and procaspase 11 and to induce apoptosis in digitonin-permeabilized cells.

Confezionamento

Package size based on protein content

Definizione di unità

One unit will liberate 1 nanomole of 7-amino-4-methylcoumarin from Z-Arg-Arg 7-amido-4-methylcoumarin per min at pH 6.0 at 40 °C.

Stato fisico

Solution in 50 mM sodium acetate, pH 5.0, with 1 mM EDTA.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable