219364

Cathepsin B, Human Liver

Cathepsin B, Human Liver, CAS 9047-22-7, is a purified native cathepsin B from human liver, purified by affinity chromatography. Upregulated in many types of tumors.

• Sinonimo/i: Cathepsin B, Human Liver
• Numero CAS: 9047-22-7
• Classificazione EC (Enzyme Commission): 3.4.22.1 (BRENDA, IUBMB)
• Numero MDL: MFCD00406994
• Codice UNSPSC: 12352202
• NACRES: NA.77

Proprietà

• Origine biologica: human liver
• Livello qualitativo: 100
• Descrizione: Merck USA index - 14, 1905
• Saggio: >95% (SDS-PAGE)
• Stato: liquid
• Attività specifica: ≥50 units/mg protein
• Purificato mediante: affinity chromatography
• Produttore/marchio commerciale: Calbiochem^®
• Condizioni di stoccaggio: OK to freeze; avoid repeated freeze/thaw cycles
• tecniche: activity assay: suitable
• Intervallo di pH: 3.5—6.0
• Compatibilità: suitable for molecular biology
• applicazioni: life science and biopharma
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: −70°C
• Informazioni sul gene: human ... CTSB(1508)

Descrizione

Descrizione generale

Research area: Cell Signaling

Cathepsin B, Human Liver, is a native cathepsin B from human liver, purified by affinity chromatography. Cathepsin B is a lysosomal protease with endo/exopeptidase[1] and a carboxydipeptidase activity. It is also known as cysteine cathepsin and belongs to the C1 family of papain-like enzymes. Cathepsin B like H, L, C, X, F, O and V is ubiquitously expressed in human tissues.[2]

Optimal pH: 3.5-6.0.

Applicazioni

Cathepsin B, Human Liver has been used to assess its linker cleavage or proteolytic activity using an LC/MS MRM-based quantitation method.[3][4]

Azioni biochim/fisiol

Cathepsin B facilitates non-specific, bulk proteolysis within the acidic conditions of the endosomal/lysosomal compartment. Here, they break down both intracellular and extracellular proteins.[2] Upregulation and secretion of cathepsin B have been shown to occur in many types of tumors and to correlate positively with their invasive and metastatic capabilities. Cathepsin B is shown to facilitate tumor invasion by dissolving extracellular barriers.

Confezionamento

Please refer to vial label for lot-specific concentration.

Attenzione

Toxicity: Standard Handling (A)

Definizione di unità

One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol Z-RR-βNA per min at 40°C, pH 6.0 in the presence of DTT.

Stato fisico

In 50 mM sodium acetate buffer, 1 mM EDTA, pH 5.0.

Nota sulla preparazione

Prepared from tissue of individuals that have been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Ricostituzione

Following initial thaw, aliquot and freeze (-70°C).

Altre note

Kostoulas, G., et al. 1999. FEBS Lett. 455, 286.
Berquim, I.M., and Sloane, B.F. 1996. Adv. Exp. Med. Biol.389, 281.
Keppler, D., et al. 1994. Biochim. Biophys. Acta.1226, 117.
Musil, D., et al. 1991. EMBO J.10, 2321.
Sloane B.F. 1990. Semin. Cancer Biol.1, 137.

Note legali

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable