12041677001

CDP-Star^®, ready-to-use

>98%, solution, suitable for dot blot, suitable for Northern blotting, suitable for Southern blotting

• Codice UNSPSC: 12352204

Proprietà

• Descrizione: Disodium 2-chloro-5-(4-methoxyspiro {1,2-dioxetane-3,2′-(5′-chloro)tricyclo[3.3.1.13,7 ]decan}-4-yl)-1-phenyl phosphate
• Livello qualitativo: 100
• Saggio: >98%
• Stato: solution
• Confezionamento: bottle of 2 × 50 mL ([0.25 mM])
• Produttore/marchio commerciale: Roche
• tecniche: Northern blotting: suitable; Southern blotting: suitable; dot blot: suitable
• Temperatura di conservazione: 2-8°C

Descrizione

Pubblicazione

Purity: Purified by HPLC.

Applicazioni

Used for the nonradioactive detection of labeled nucleic acid probes on membrane blots:

• Southern blot[1]
• Northern blot[2]
• Dot blot
• Colony and plaque hybridization
• Gel shift assays

Used for the nonradioactive detection of labeled nucleic acid probes on membrane blots:

• Southern blot [3][1]
• Northern blot[4][2]
• Dot blot[5]
• Colony and plaque hybridization
• Gel shift assays
• Chemiluminescent detection.[6]

Caratteristiche e vantaggi

Contents
0.25 mM solution (0.124 mg/ml), ready-to-use, colorless solution

CDP-Star is a chemiluminescent substrate for alkaline phosphatase that enables fast, ultra-sensitive detection of biomolecules by producing extremely rapid visible light. Light emission is recorded on X-ray film or on luminescence imager systems.

• Save time with a fast, ultra-sensitive substrate.
• Easily strip and reprobe membranes.
• Take multiple exposures of up to two days for each experiment.

Reuse the substrate by filtering and storing in sodium azide.

Nota sulla preparazione

Storage conditions (working solution): Washing Buffer: 15 to 25 °C, stable
Maleic Acid Buffer: 15 to 25 °C, stable
Detection Buffer: 15 to 25 °C, stable
Blocking Solution: always prepare freshly
Antibody Solution: 2 to 8 °C

Altre note

• First exposure to X-ray film should be 5-15 minutes. Examine film and then determine other exposure times based on initial result.
• No preincubation step required.
• Apply substrate quickly to avoid gray shadows from substrate drops and drying of membrane (leads to uneven, high background).
• Do not use plastic wrap to cover blot; use hybridization bags, acetate sheet protectors, or two sheets of transparent film.
• Exposure times are shorter than with CSPD.
• Do not use nitrocellulose membranes.

Repeat exposures can be made up to two days after the addition of substrate.

For life science research only. Not for use in diagnostic procedures.

Note legali

CDP-Star is a registered trademark of Tropix, Inc.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): nwg
• Punto d’infiammabilità (°F): does not flash
• Punto d’infiammabilità (°C): does not flash