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C6854

Sigma-Aldrich

Cathepsin L from human liver

≥0.5 units/mg protein, solution

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25 μG
CHF 650.00

CHF 650.00

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25 μG
CHF 650.00

About This Item

Numero CAS:
60616-82-2
Classificazione EC (Enzyme Commission):
3.4.22.15 (BRENDA, IUBMB)
Numero MDL:
MFCD00163235
Codice UNSPSC:
12352200
NACRES:
NA.32

CHF 650.00

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Origine biologica

human liver

Livello qualitativo

200

Stato

solution

Attività specifica

≥0.5 units/mg protein

tecniche

activity assay: suitable

Intervallo di pH

4.5—5.5

N° accesso UniProt

P07711

applicazioni

pharmaceutical

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

Informazioni sul gene

human ... CTSL1(1514)

Descrizione generale

The cathepsin L (CTSL) gene is mapped to human chromosome 9q21.33. It encodes a lysosomal cysteine proteinase that belongs to the peptidase C1 family. The enzyme is a dimer of a heavy (175 amino acids) and a light chain (42 amino acids) linked by disulfide bonds.

Applicazioni

Cathepsin L (CTSL) from human liver has been used:
  • to digest mutant transforming growth factor β-induced (TGFBIp) and LC3 (autophagosomes marker) proteins in vitro
  • to digest the pH-dependent fibril of a pre-melanosomal protein (Pmel17) repeat domain (RPT) isoform and study the effect of pH on sRPT aggregation kinetics by tryptophan fluorescence
  • to analyze the inhibition of CTSL by small molecules or drugs in a cell-free system

Azioni biochim/fisiol

Cathepsin L (CTSL) exhibits endopeptidase activity and breaks peptide bonds with aromatic residues. This enzyme is functional at pH 3.0–6.5 with thiol compounds. Elastin, collagen, and α-1 protease inhibitors act as substrates for cathepsin L (CTSL). CTSL cleaves several proteins including enzymes, receptors, and transcription factors. It is involved in antigenic peptide production and controls B-cells homeostasis. It is associated with myofibril necrosis and tumor progression. Increased expression of CTSL promotes metastasis and relates to poor prognosis in cancer patients. Increased expression of CTSL is observed in breast cancer. CTSL is implicated in glioblastoma multiforme (GBM), middle east respiratory syndrome (MERS), gingival overgrowth, and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). It has a higher specific activity than cathepsin B and H in the degradation of a variety of physiological protein substrates.

Definizione di unità

One unit will hydrolyze 1.0 μmole of Z-Phe-Arg-AFC per minute at pH 5.5 at 25 °C.

Stato fisico

Solution in in 20 mM malonate, pH 5.5, 1 mM EDTA, and 400 mM NaCl.

Prodotti correlati

N° Catalogo
Descrizione
Determinazione del prezzo

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

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Certificati d'analisi (COA)

Lot/Batch Number
0000266834
0000266834
0000264664
0000264664
SLCR2105

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Seung-Il Choi et al.
Journal of cellular and molecular medicine, 24(18), 10343-10355 (2020-07-16)
Granular corneal dystrophy type 2 (GCD2) is the most common form of transforming growth factor β-induced (TGFBI) gene-linked corneal dystrophy and is pathologically characterized by the corneal deposition of mutant-TGFBIp. The defective autophagic degradation of pathogenic mutant-TGFBIp has been shown
Bing-Jie Lv et al.
Atherosclerosis, 230(1), 100-105 (2013-08-21)
Cathepsin L (CatL), cathepsin K (CatK), and cathepsin V (CatV) are potent elastases implicated in human arterial wall remodeling. Whether plasma levels of these cathepsins are altered in patients with abdominal aortic aneurysms (AAAs) remains unknown. Plasma samples were collected
Dexter N Dean et al.
Biochimica et biophysica acta. Proteins and proteomics, 1867(10), 961-969 (2019-02-05)
The pre-melanosomal protein (Pmel17) aggregates within melanosomes to form functional amyloid fibrils that facilitate melanin polymerization. The repeat domain (RPT) of Pmel17 fibrillates under strict acidic melanosomal pH. Alternative splicing results in a shortened repeat domain (sRPT), which also forms
Bailey Miller et al.
Journal of natural products, 77(1), 92-99 (2013-12-25)
A number of marine natural products are potent inhibitors of proteases, an important drug target class in human diseases. Hence, marine cyanobacterial extracts were assessed for inhibitory activity to human cathepsin L. Herein, we have shown that gallinamide A potently
Violeta Morin et al.
PloS one, 7(11), e46850-e46850 (2012-11-13)
Proteolysis of sperm histones in the sea urchin male pronucleus is the consequence of the activation at fertilization of a maternal cysteine protease. We previously showed that this protein is required for male chromatin remodelling and for cell-cycle progression in
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Questions

  1. Hello, What is the protocol to bring this up in solution to have a concentration of 0.5 units/µL? Does units mean µg where the amount needed for performing digestions? Is there a protocol associated with this?

    1 answer

    1. This product is supplied as a solution. The minimum activity for each lot will be ≥0.5 units/mg protein. The specific activity of each lot is reported on the Certificate of Analysis. See the link below to review a sample Certificate:
      https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/C6854-25UGSLCR2105.pdf

      The product is supplied in 20 mM malonate buffer, pH 5.5, with 1 mM EDTA and 400 mM NaCl. One unit is defined as the amount of Cathepsin L that hydrolyzes 1 uM Z-PHE-Arg-AFC per minute at 22 - 25 °C, in 400 mM Sodium Acetate, pH 5.5, containing 4 mM EDTA and 8 mM DTT.

      Please access the product datasheet at the link below:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/130/380/c6854dat.pdf

      Helpful?

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