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C6788

Sigma-Aldrich

4-Chloro-1-naphthol

peroxidase substrate, tablet, suitable for use in immunoblotting procedures

Sinonimo/i:

4-CN, 4-chloronaphthalen-1-ol

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About This Item

Formula condensata:
ClC10H6OH
Numero CAS:
Peso molecolare:
178.61
Beilstein:
509750
Numero CE:
Numero MDL:
Codice UNSPSC:
12352204
ID PubChem:
NACRES:
NA.83

product name

4-Chloro-1-naphthol, tablet

Forma fisica

tablet

Punto di fusione

118-121 °C (lit.)

Solubilità

methanol: 10 mL, clear, colorless

Temperatura di conservazione

−20°C

Stringa SMILE

Oc1ccc(Cl)c2ccccc12

InChI

1S/C10H7ClO/c11-9-5-6-10(12)8-4-2-1-3-7(8)9/h1-6,12H
LVSPDZAGCBEQAV-UHFFFAOYSA-N

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Specificità

4-Chloro-1-Naphthol is a peroxidase substrate suitable for use in immunoblotting procedures. This substrate produces an insoluble end product that is blue in color and can be observed visually.

Applicazioni

4-Chloro-1-naphthol has been used as a substrate in enzyme-linked immunospot (ELISpot) assay for detection of the spots. It has also been used as a precipitating chromogenic substrate for visualizing the antigen−antibody-reactive bands following western blotting.

Quantità

Contains 30 mg substrate per tablet.

Ricostituzione

Dissolve 1 tablet in 10 ml of methanol. Add 2 ml of methanol stock solution to 10 ml of triethanolamine buffered saline, pH 7.5. Add 5 μl of fresh 30% hydrogen peroxide (Sigma Product No. H-1009) immediately prior to use.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

dust mask type N95 (US), Eyeshields, Gloves


Certificati d'analisi (COA)

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NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

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