C8302

4-Chloro-1-naphthol solution

Name: 4-Chloro-1-naphthol solution
Brand: SIGMA
Price: 125 CHF

For HRP detection on Western Blots

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100 ML

CHF 125.00

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Informazioni su questo articolo

Numero CAS:

604-44-4

NACRES:

NA.52

PubChem Substance ID:

24893047

UNSPSC Code:

12352200

MDL number:

MFCD00003974

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InChI

1S/C10H7ClO/c11-9-5-6-10(12)8-4-2-1-3-7(8)9/h1-6,12H

SMILES string

Oc1ccc(Cl)c2ccccc12

InChI key

LVSPDZAGCBEQAV-UHFFFAOYSA-N

grade

Molecular Biology

form

liquid

storage temp.

2-8°C

Quality Level

200

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Questo articolo	N7660	185507	N2125
Sigma-Aldrich C8302 4-Chloro-1-naphthol solution	Sigma-Aldrich N7660 4-Nitrophenol solution	Sigma-Aldrich 185507 2-Naphthol	Sigma-Aldrich N2125 Naphthol AS-BI phosphate
grade for molecular biology	grade -	grade -	grade -
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
form liquid	form liquid	form powder or flakes	form powder
storage temp. 2-8°C	storage temp. 2-8°C	storage temp. -	storage temp. −20°C

General description

4-Chloro-1-naphthol is a substrate for HRP in a reaction that results in a colored precipitate. It is commonly used for colorimetric detection and visualization of proteins in western blotting.

Application

Suitable for visualizing protein-peroxidase conjugates in western blotting.

Biochem/physiol Actions

Enzymes such as HRP convert 4-Chloro-1-naphthol to a purple-colored precipitate on the blot that is readily visible to the eye.

Other Notes

0.48 mM 4-chloro-1-naphthol, 50 mM Tris-HCl, 0.2 M NaCl and 17% methanol.

This product contains 0.48 mM 4-chloro-1-naphthol, 50 mM Tris-HCl, 0.2 M NaCl and 17% methanol.

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Descrizione

Determinazione del prezzo

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Ponceau S solution, BioReagent, suitable (for use in cellulose acetate electrophoresis), 0.1 % (w/v) in 5% acetic acid

P7059

Phosphate Buffered Saline, 10× PBS for Western blots and IP

RPROB

Reversible Protein Detection Kit for Membranes and Polyacrylamide Gels

W0138

Western Blocker^™ Solution, for HRP detection systems

SDS7B2

Prestained Molecular Weight Marker, mol wt 26,600-180,000 Da

C1992

ColorBurst^™ Electrophoresis Marker, mol wt 8,000-220,000 Da

GERPN2209

ECL^™ Western Blotting Detection Reagents, Cytiva RPN2209

pictograms

GHS08,GHS07

signalword

Danger

hcodes

H302 + H312 + H332,H370

pcodes

P260 - P280 - P301 + P312 - P302 + P352 + P312 - P304 + P340 + P312 - P308 + P311

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - STOT SE 1

target_organs

Eyes,Central nervous system

Classe di stoccaggio

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 2

flash_point_f

163.9 °F - closed cup

flash_point_c

73.3 °C - closed cup

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

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Colorimetric Detection of Western blot using 4-Chloro-1-Naphthol Solution Protocol

This solution is used as a substrate for detecting horseradish-peroxidase conjugates in Western blotting.

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

Contenuto correlato

Data Sheet - C8302

Datasheet

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