Product A9594, Monoclonal ANTI-FLAG® M2-Cy3™ antibody produced in mice, is mouse IgG1. Theoretically, it will be recognized by the anti-mouse IgG secondary antibody. However, Product A9594 is already conjugated with Cy3 so a secondary antibody is not needed for detection.
A9594
Anticorpo monoclonale ANTI-FLAG® M2
clone M2, purified immunoglobulin, buffered aqueous solution (Supplied as a solution in 10 mM sodium phosphate)
Sinonimo/i:
Anti-ddddk, Anti-dykddddk
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0.2 MG
CHF 621.00
1 MG
CHF 1’500.00
5 X 1 MG
CHF 2’140.00
CHF 621.00
Spedizione prevista il02 giugno 2025
Scegli un formato
Cambia visualizzazione
0.2 MG
CHF 621.00
1 MG
CHF 1’500.00
5 X 1 MG
CHF 2’140.00
About This Item
Codice UNSPSC:
41106514
NACRES:
NA.32
CHF 621.00
Spedizione prevista il02 giugno 2025
Prodotti consigliati
Origine biologica
mouse
Coniugato
CY3 conjugate
Forma dell’anticorpo
purified immunoglobulin
Tipo di anticorpo
primary antibodies
Clone
M2, monoclonal
Stato
buffered aqueous solution (Supplied as a solution in 10 mM sodium phosphate)
Reattività contro le specie
all
Concentrazione
~1 mg/mL
tecniche
direct immunofluorescence: 10 μg/mL using mammalian cells fixed with methanol:acetone
Isotipo
IgG1
Sequenza immunogenica
DYKDDDDK
Condizioni di spedizione
dry ice
Temperatura di conservazione
−20°C
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Categorie correlate
Descrizione generale
Monoclonal ANTI-FLAG M2-Cy3 (mouse IgG) antibody is covalently conjugated to cyanine dye Cy3. The antibody conjugate binds to FLAG fusion proteins, and will recognize the FLAG sequence at the N-terminus, Met-N-terminus, or C-terminus of FLAG fusion proteins.
Applicazioni
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Western Blotting (1 paper)
For simple, one-step detection by immunocytochemistry. Especially useful in detection of FLAG fusion proteins expressed in murine host, where secondary anti-mouse antibodies might cause cross-reactivity.
Learn more product details in our FLAG® application portal.
Learn more product details in our FLAG® application portal.
Stato fisico
Solution in phosphate buffered saline plus 1% BSA and preservative
Nota sulla preparazione
Dilute the antibody in Tris buffered saline (TBS): 0.05 M Tris,pH 7.4, with 0.15 M NaCl.
Altre note
Suggested concentration of 1-10 mg/ml for immunocytochemistry.
Note legali
ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Cy3 is a trademark of Cytiva
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
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Codice della classe di stoccaggio
10 - Combustible liquids
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
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A9594（FLAG M2 Cy3標識）の抗体は抗マウスIgGの2次抗体に認識されますか。
1 answer-
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