The "1mL" indicates that this product is supplied at a volume of 1 milliliter. This ANTI-FLAG M2 affinity gel is supplied as a 50% suspension in 50% glycerol with 10 mM sodium phosphate and 150 mM sodium chloride, pH 7.4, containing 0.02% (w/v) sodium azide (PBS/A).
A2220
Gel d′affinità ANTI-FLAG® M2
ANTI-FLAG® M2 Affinity Gel
purified immunoglobulin, buffered aqueous glycerol solution
Sinonimo/i:
Anticorpo monoclonale ANTI-FLAG® M2, Gel d’affinità ANTI-FLAG® M2 in agarosio, Anti-ddddk, Anti-dykddddk
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1 ML
CHF 488.00
5 ML
CHF 1’520.00
10 ML
CHF 2’380.00
25 ML
CHF 5’020.00
2 X 25 ML
CHF 7’830.00
4 X 25 ML
CHF 15’140.00
Scegli un formato
Cambia visualizzazione
1 ML
CHF 488.00
5 ML
CHF 1’520.00
10 ML
CHF 2’380.00
25 ML
CHF 5’020.00
2 X 25 ML
CHF 7’830.00
4 X 25 ML
CHF 15’140.00
About This Item
Codice UNSPSC:
12352203
NACRES:
NA.32
Clone:
M2, monoclonal
application:
IP
affinity chromatography
affinity chromatography
tecniche:
affinity chromatography: suitable
immunoprecipitation (IP): suitable
immunoprecipitation (IP): suitable
citations:
2532
Prodotti consigliati
Coniugato
agarose conjugate
Livello qualitativo
Forma dell’anticorpo
purified immunoglobulin
Tipo di anticorpo
primary antibodies
Clone
M2, monoclonal
Stato
buffered aqueous glycerol solution
Classi chimiche degli analiti
proteins
tecniche
affinity chromatography: suitable
immunoprecipitation (IP): suitable
Matrice
(4% agarose bead; 45-165μm bead size)
Isotipo
IgG1
Capacità
>0.6 mg/mL, resin binding capacity (FLAG-BAP)
Condizioni di spedizione
wet ice
Temperatura di conservazione
−20°C
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Descrizione generale
Il gel di affinità Anti-FLAG M2 consiste di un anticorpo monoclonale di topo legato covalentemente all′agarosio. L′anticorpo lega il peptide FLAG in posizione N-terminale, Met-N-terminale, C-terminale o in corrispondenza di posizioni interne delle proteine di fusione. Il legame è calcio-indipendente
Eluizione - peptide FLAG®, Glicina, pH 3,5, peptide FLAG® 3x
Eluizione - peptide FLAG®, Glicina, pH 3,5, peptide FLAG® 3x
FLAG® peptide, Glycine, pH3.5, 3x FLAG® peptide
Immunogeno
DYKDDDDK
Applicazioni
Il gel di affinità Anti-FLAG® M2 è stato utilizzato per western blotting, immunoprecipitazione e per la purificazione di proteine di fusione con tag FLAG.[1][2][3]
Esplora tutti i dettagli di prodotto sul nostro portale dedicato alle applicazioni FLAG®.
Esplora tutti i dettagli di prodotto sul nostro portale dedicato alle applicazioni FLAG®.
Stato fisico
Sospensione in tampone contenente azide come conservante e glicerolo al 50%.
Note legali
ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Esclusione di responsabilità
Tag FLAG™, 3x FLAG™, tag DYKDDDDK
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Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
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Certificati d'analisi (COA)
Lot/Batch Number
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MicroRNAs (miRNAs) bind to Argonaute proteins, and together they form the RISC complex and regulate target mRNA translation and/or stability. Identification of mRNA targets is key to deciphering the physiological functions and mode of action of miRNAs. In mammals, miRNAs
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Ca(2+)/calmodulin-dependent protein kinase kinase β (CaMKKβ) is a serine/threonine-directed kinase that is activated following increases in intracellular Ca(2+). CaMKKβ activates Ca(2+)/calmodulin-dependent protein kinase I, Ca(2+)/calmodulin-dependent protein kinase IV, and the AMP-dependent protein kinase in a number of physiological pathways, including
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Articoli
The FLAG® Expression System is a proven method to express, purify and detect recombinant fusion proteins. Sigma®, the proven provider of FLAG®, now offers a magnetic bead for immunoprecipitation, protein purification, and the study of protein-protein interactions. The ANTI-FLAG® M2 Magnetic Bead is composed of murine derived, anti-FLAG® M2 monoclonal antibody attached to superparamagnetic iron impregated 4% agarose beads, with an average diameter of 50 µm. The M2 antibody is capable of binding to fusion proteins containing a FLAG peptide sequence at the N-terminus, Met-N-terminus, or C-terminus locations in mammalian, bacterial, and plant extracts.
Protocolli
Protocol for immunoprecipitation (IP) of FLAG fusion proteins using M2 monoclonal antibody 4% agarose affinity gels
Contenuto correlato
Protein purification techniques, reagents, and protocols for purifying recombinant proteins using methods including, ion-exchange, size-exclusion, and protein affinity chromatography.
Tecniche, reagenti e protocolli per la purificazione di proteine ricombinanti mediante tecniche di cromatografia a scambio ionico, di esclusione dimensionale e di affinità.
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May I ask a question about the product? Does "1mL" mean 1 mL Slurry plus 50% glycerol, which is 2 mL in total? Same as other (e.g., 10 mL = 50% slurry with total 20 mL volume)
1 answer-
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What is the bead size for this product?
1 answer-
As stated in the 'PROPERTIES' section of the product page, the bead size range is 45-165 μm.
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Is there a generic agarose bead available for purchase that can be used as a control to test for non-specific binding, in the case that something is binding to the agarose bead itself rather than the tag? If it is not listed on the site, is there a possibility of obtaining generic agarose beads through alternative means? Alternatively, could any information be provided to determine if another provider's generic agarose beads are produced in a similar manner for this specific purpose?
1 answer-
Yes, there are two options available for a generic agarose bead as a control to test for non-specific binding. One option is Product Number. 4B200, Sepharose® 4B, which can be used for sample preclearing and is equivalent to the beads in the FLAG affinity product. The other option is Product Number: A0919, Mouse IgG−Agarose, which can serve as a conjugated antibody bead control.
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Is there a protocol for regeneration?
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Please see the link below to the product data-sheet. Instructions state that the column should be regenerated immediately after use by washing with three column volumes of 0.1 M glycine HCI, pH 3.5. The column should be immediately re-equilibrated in TBS until the effluent is at neutral pH:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/288/183/a2220bul-ms.pdf
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When using ANTI-FLAG M2 Affinity Gel, Product A2220, should I use a 3X FLAG peptide or a 1X FLAG peptide to elute my protein?
1 answer-
If you have a 3X FLAG-tagged protein, then you will need to use the 3X FLAG peptide. If you have a 1X FLAG-tagged protein, you can use the 1X FLAG peptide or the 3X FLAG peptide. We have not noticed a significant difference in elution efficiency by using a 3X FLAG peptide on a 1X FLAG-tagged protein.
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How should I elute my protein when using Product No. A2220, ANTI-FLAG® M2 Affinity Gel?
1 answer-
Elution with the peptide is the most gentle method. Acid elution (0.1 M glycine-HCL pH 3.5) is a more stringent method of elution, and should be evaluated for its effect on your protein if it is to be used in downstream applications. Boiling the resin in sample buffer is the most denaturing condition. If this condition is used, the resin cannot be re-used, due to the presence of SDS and/or reducing agents. The elution information can be viewed on A2220 product information sheet (under Documents, above).
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I am using Product No. A2220, ANTI-FLAG® M2 Affinity Gel, and have a lot of non-specific proteins that are eluting with my FLAG®-tagged protein. How can I get rid of these?
1 answer-
The product bulletin for Product A2220, ANTI-FLAG® M2 affinity gel indicates:Pre-clear lysate with Mouse IgG-Agarose (Product A0919) to remove nonspecific binding proteins. Alternatively, you can use the unconjugated resin (Product 4B200) for this purpose. Other methods to remove non-specific binding from the resin would be to increase the stringency of the washes by increasing salt concentration (the resin can tolerate up to 1M NaCl) or including detergents that are compatible with the resin.
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When using Product No. A2220, ANTI-FLAG® M2 Affinity Gel, I see bands at 20-25 kDa and 50-60 kDa appearing in my Westerns that are not my FLAG®-tagged protein. How can I prevent this?
1 answer-
As a result of the conjugation, there may be some M2 antibody that is not conjugated to the resin, but is associated with the resin and may appear in acid elutions as heavy and light chain when using the anti-mouse IgG conjugated secondary antibody. We recommend an acid wash (0.1 M glycine-HCL pH 3.5) and neutralization of the resin (do not allow the acid wash to sit on the resin longer than 20 minutes) prior to applying the lysate. Another way to avoid this is to use a directly conjugated FLAG® antibody for detection such as product A8592 ANTI-FLAG® M2 HRP, or the rabbit anti-FLAG® polyclonal antibody, F7425.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Is my lysis buffer compatible with Product No. A2220, ANTI-FLAG® M2 Affinity Gel?
1 answer-
The A2220 product information sheet (under Documents, above) contains a reagent compatibility table located on page 6. We do not recommend addition of SDS or reducing agents such as DTT, DTE or 2-mercaptoethanol to the resin. If you have a detergent listed in the table in a higher than recommended concentration, we recommend trying to dilute the sample before applying to the resin.
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