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A8011

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Antifoam C Emulsion

aqueous-silicone emulsion

Sinonimo/i:

Antifoaming Agent Emulsion

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About This Item

Codice UNSPSC:
12161900
NACRES:
NA.25

Origine biologica

synthetic

Forma fisica

emulsion

contiene

emulsifier

tecniche

cell culture | hybridoma: suitable
microbiological culture: suitable

Punto di fusione

0  °C (32 °F)

Descrizione generale

Antifoam C emulsion is an aqueous emulsion containing 30% active silicone. This silicone-based antifoam emulsion consists of non-ionic emulsifiers different from those in antifoam emulsions B and Y-30. Antifoam C emulsion can be prediluted with 3-10 parts of cool water to aid in dispersion. Prediluted suspensions should be used immediately. Antifoam C emulsion is typically effective at 1-10 ppm. The flow properties of antifoam C emulsion are such that it can be pumped on an as-needed basis to a fermenter system with sufficient agitation to disperse the antifoam. Antifoam C emulsion can be used to regulate headspace foaming in mammalian cell culture bioreactors up to 30 ppm concentration.

Applicazioni

Antifoam C emulsion has been used:
  • as a supplement in RO-H2O and a chemically defined culture medium to determine volumetric mass transfer coefficient (kLa) of mammalian cell culture
  • as a component of feed medium,
  • as a steam-sterilized antifoaming agent in a biocontroller for pH-stat fed-batch cultivations
  • as a supplement in fed batch medium to mimic the typical large-scale cultivation conditions

Caratteristiche e vantaggi

  • Minimizes compatibility issues with biological systems
  • Highly effective defoamer at low concentrations
  • Highly effective and versatile silicone defoamer

Altre note

A 30% aqueous emulsion of polydimethylsiloxane concentrate.
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Pittogrammi

Exclamation mark

Avvertenze

Warning

Indicazioni di pericolo

Classi di pericolo

Eye Irrit. 2 - STOT SE 3

Organi bersaglio

Respiratory system

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type ABEK (EN14387) respirator filter


Certificati d'analisi (COA)

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In aerobic cell cultivation processes, dissolved oxygen is a key process parameter, and an optimal oxygen supply has to be ensured for proper process performance. To achieve optimal growth and/or product formation, the rate of oxygen transfer has to be
Bernhard Sissolak et al.
Biotechnology journal, 14(7), e1800714-e1800714 (2019-04-16)
Frequently measured mammalian cell culture process indicators include viability and total cell concentration (TCC). Cell lysis, an additional important process characteristic that substantially contributes to the overall product purity profiles, is often not addressed in detail. In the present study
Gang Fu et al.
Biotechnology and applied biochemistry, 67(5), 812-818 (2019-10-08)
d-Psicose 3-epimerase is an enzyme that catalyzes the synthesis of d-psicose from d-fructose. We cloned the d-psicose 3-epimerase from Ruminococcus sp. (RDPE) and expressed it in Bacillus subtilis A311. By a two-step pH regulation of segmented fermentation, we significantly improved
Mervyn W O Liew et al.
Journal of biotechnology, 150(2), 224-231 (2010-08-28)
This study demonstrates the feasibility of large-scale production of murine polyomavirus VP1 protein in recombinant Escherichia coli as pentamers which are able to subsequently self-assemble in vitro into virus-like particles (VLPs). High-cell-density pH-stat fed-batch cultivation was employed to produce glutathione-S-transferase
Characterizing the Effects of Antifoam C Emulsion on Oxygen Mass Transfer within the BIOne Drilled-Hole Sparger and Microsparger Single-Use Bioreactor Systems
McAndrew J and Kauffman G
American Pharmacy null

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