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A6154

Sigma-Aldrich

Anti-Rabbit IgG (whole molecule)–Peroxidase antibody produced in goat

affinity isolated antibody, buffered aqueous solution

Sinonimo/i:

Goat Anti-Rabbit IgG (whole molecule)–HRP

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1 ML
CHF 156.00
5 X 1 ML
CHF 507.00

CHF 156.00


Spedizione prevista il17 aprile 2025


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Cambia visualizzazione
1 ML
CHF 156.00
5 X 1 ML
CHF 507.00

About This Item

Numero MDL:
Codice UNSPSC:
12352203
NACRES:
NA.46

CHF 156.00


Spedizione prevista il17 aprile 2025


Richiedi un ordine bulk

Origine biologica

goat

Livello qualitativo

Coniugato

peroxidase conjugate

Forma dell’anticorpo

affinity isolated antibody

Tipo di anticorpo

secondary antibodies

Clone

polyclonal

Stato

buffered aqueous solution

Reattività contro le specie

rabbit

tecniche

direct ELISA: 1:10,000

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

modifica post-traduzionali bersaglio

unmodified

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Descrizione generale

IgGs are glycoprotein antibodies that modulate several immune responses. Rabbit IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-rabbit IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins. Goat Anti-Rabbit IgG (whole molecule)-Peroxidase antibody is immunospecific for rabbit IgG by immunoelectrophoresis against normal serum and rabbit IgG, prior to conjugation.

Immunogeno

purified rabbit IgG

Applicazioni

C33A cell lysates were analyzed by western blot using HRP-conjugated goat anti-rabbit IgG as the secondary antibody. Protein aliquots from colonic mucosal extracts (μg) were anlayzed by western blot using HRP-conjugated goat anti-rabbit IgG as the secondary antibody at a 1:5000 dilution in TBSt for 1 hr at room temperature.
Co-immunoprecipation and western blot analysis of C33A cell lysates were performed using HRP conjugated goat anti-rabbit IgG as the secondary antibody.
Goat Anti-Rabbit IgG (whole molecule)-Peroxidase antibody has been used for western blot (1:6,000), and ELISA assays.

Stato fisico

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin with preservative.

Nota sulla preparazione

Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978).

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Pittogrammi

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Avvertenze

Warning

Indicazioni di pericolo

Classi di pericolo

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


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