A4914
Anti-Rabbit IgG (whole molecule)–Peroxidase antibody produced in goat
affinity isolated antibody, buffered aqueous solution
Sinonimo/i:
Goat Anti-Rabbit IgG (whole molecule)–HRP
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About This Item
Prodotti consigliati
Origine biologica
goat
Coniugato
peroxidase conjugate
Forma dell’anticorpo
affinity isolated antibody
Tipo di anticorpo
secondary antibodies
Clone
polyclonal
Forma fisica
buffered aqueous solution
tecniche
direct ELISA: 1:10,000
applicazioni
research pathology
Condizioni di spedizione
dry ice
Temperatura di conservazione
−20°C
modifica post-traduzionali bersaglio
unmodified
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Categorie correlate
Descrizione generale
IgGs are glycoprotein antibodies that modulate several immune responses. Rabbit polyclonal IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary polyclonal anti-rabbit IgGs conjugated to a detectable substrate are useful tools for the immunological analysis of target proteins. Anti-Rabbit IgG (whole molecule)-Peroxidase antibody is specific for all rabbit IgGs and does not bind to human serum proteins in ELISA.
Immunogeno
Purified rabbit IgG
Applicazioni
Anti-Rabbit IgG (whole molecule)-Peroxidase antibody can be used in sandwich ELISA (diluted 1:3000 in PBS-0.05% Tween 20) and immunoelectrophoresis. The product can also be used for western blotting applications (at 1:2000 dilutions in mouse liver homogenates , or in mouse embryonic fibroblasts ).
Co-immunoprecipation and western blot analysis of C33A cell lysates were performed using HRP conjugated goat anti-rabbit IgG as the secondary antibody.
Altre note
Antibody adsorbed with human serum proteins.
Stato fisico
Solution in 0.01 M phosphate buffered saline, pH 7.4 containing 1% bovine serum albumin with preservative.
Nota sulla preparazione
Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978).
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Avvertenze
Warning
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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