A0545

Anti-Rabbit IgG (whole molecule)–Peroxidase antibody produced in goat

affinity isolated antibody

• Sinonimo/i: Anti Rabbit, Anti Rabbit Antibody, Anti Rabbit Igg, Anti-Rabbit Igg, Goat Anti Rabbit IgG Antibody - Anti-Rabbit IgG (whole molecule)–Peroxidase antibody produced in goat, Goat Anti Rabbit Igg
• Numero MDL: MFCD00162788
• Codice UNSPSC: 12352203
• NACRES: NA.46

Proprietà

• Origine biologica: goat
• Livello qualitativo: 300
• Coniugato: peroxidase conjugate
• Forma dell’anticorpo: affinity isolated antibody
• Tipo di anticorpo: secondary antibodies
• Clone: polyclonal
• Reattività contro le specie: rabbit
• Non deve reagire con: human
• tecniche: direct ELISA: 1:30,000 using using 5 μg/ml of rabbit IgG for the coating and OPD substrate; immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200; western blot (chemiluminescent): 1:80,000-1:160,000 using detecting β-actin in total cell extract of HeLa cells (5-10 μg/mL)
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −20°C
• modifica post-traduzionali bersaglio: unmodified

Descrizione

Descrizione generale

Goat anti-rabbit IgG-peroxidase conjugate associates with all rabbit Igs. The conjugate shows no reaction with human IgG by immunoelectrophoresis (IEP). Furthermore, the antibody is adsorbed with human IgG to reduce background staining with human samples.

Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It is a glycoprotein and mainly helps in immune defense. IgG is a major class of immunoglobulin. Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. Rabbit IgG is further divided into five classes- IgM, IgG, IgA, IgD and IgE. It has only one IgG variant with one interheavy disulfide bridge. It helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.

Specificità

Specificity of the Anti-Rabbit IgG-Peroxidase is determined by immunoelectrophoresis (IEP) using normal rabbit serum and rabbit IgG. The conjugate shows no reaction with human IgG by IEP.

Immunogeno

Purified rabbit IgG

Applicazioni

Anti-Rabbit IgG (whole molecule)–Peroxidase antibody has been used in western blotting , immunofluorescence staining , immunochemistry and immunoprecipitations.

Co-immunoprecipation and western blot analysis of C33A cell lysates were performed using HRP conjugated goat anti-rabbit IgG as the secondary antibody.

Goat anti-rabbit IgG conjugated to peroxidase can be used for direct ELISA and immunohistochemistry.

Peroxidase-conjugated goat anti-rabbit IgG antibody was used for western blot analysis of Cos 7 cell lysates at a 1:2000 dilution and yeast lysates following protein carbonylation assays at a dilution of 1:5000.
Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in goat has been used as a secondary antibody during immunofluorescence staining.

Azioni biochim/fisiol

IgGs (immunoglobulins) modulate several immune responses.It mainly helps in immune defense. Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. It helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.

Stato fisico

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT

Nota sulla preparazione

Prepared by two-step glutaraldehyde method.

Stoccaggio e stabilità

For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Altre note

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Pittogrammi: GHS07
• Avvertenze: Warning
• Indicazioni di pericolo: H317
• Consigli di prudenza: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Classi di pericolo: Skin Sens. 1
• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable