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A5278

Sigma-Aldrich

Anti-Mouse IgG (whole molecule)–Peroxidase antibody produced in goat

IgG fraction of antiserum, buffered aqueous solution

Sinonimo/i:

Anti Mouse Hrp Sigma

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About This Item

Numero MDL:
Codice UNSPSC:
12352203
NACRES:
NA.46

Origine biologica

goat

Coniugato

peroxidase conjugate

Forma dell’anticorpo

IgG fraction of antiserum

Tipo di anticorpo

secondary antibodies

Clone

polyclonal

Forma fisica

buffered aqueous solution

tecniche

direct ELISA: 1:10,000

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

modifica post-traduzionali bersaglio

unmodified

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Descrizione generale

IgG antibodies are the smallest antibody isotype found in blood and extracellular fluid. These antibodies are capable of fighting bacterial and viral infection. They also regulate several functions like placental transport, cell surface-receptor binding and complement activation. Anti-mouse IgG (whole molecule)-peroxidase antibody can be used in ELISA (diluted 1: 200 in PBST) . It can also be used in western blotting. Goat anti-mouse IgG-peroxidase antibody reacts specifically with mouse IgA, IgG1, IgG2a, IgG2b, IgG3 and IgM myeloma proteins.

Immunogeno

Purified mouse IgG.

Applicazioni

Anti-mouse IgG (whole molecule)-peroxidase antibody (diluted 1: 300) can be used as a secondary antibody in immunohistochemistry. It can also be used in Ouchterlony double diffusion and immunoelectrophoresis.
Western blot analysis using HRP conjugated goat anti-mouse IgG as the secondary antibody at a dilution of 1:3000 in BSA/PBS was performed to detect IgG antibodies against outer-membrane vexicle proteins.

Stato fisico

Solution in 0.01 M phosphate buffered saline, pH 7.4 containing 1% bovine serum albumin with preservative.

Nota sulla preparazione

Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978).

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Pittogrammi

Exclamation markEnvironment

Avvertenze

Warning

Indicazioni di pericolo

Classi di pericolo

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


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