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SRP0252

Sigma-Aldrich

ULK Active human

recombinant, expressed in FreeStyle™ 293-F cells, ≥40% (SDS-PAGE)

Synonym(e):

ATG1, ATG1 autophagy related 1 homolog, Serine/threonine-protein kinase ULK1, UNC-51-like kinase

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About This Item

UNSPSC-Code:
12352200
NACRES:
NA.32

Biologische Quelle

human

Rekombinant

expressed in FreeStyle™ 293-F cells

Assay

≥40% (SDS-PAGE)

Form

aqueous solution

Mol-Gew.

113 kDa

Verpackung

pkg of 10 μg

Hersteller/Markenname

Invitrogen

Lagerbedingungen

avoid repeated freeze/thaw cycles

Konzentration

>0.02 mg/mL

NCBI-Hinterlegungsnummer

NM_003565

UniProt-Hinterlegungsnummer

O75385

Versandbedingung

dry ice

Lagertemp.

−70°C

Angaben zum Gen

human ... ULK1(8408)

Allgemeine Beschreibung

ULK1 (unc-51 like autophagy activating kinase 1) is a Ser/Thr protein kinase which forms a component of the ULK1 protein kinase complex. This protein complex also contains the adaptor proteins ATG (autophagy-related) 13, RB1CC1/FIP200 (RB1-inducible coiled-coil 1), and ATG101. ULK1 protein is one of the five orthologs of yeast Atg1 found in mammals.

Biochem./physiol. Wirkung

ULK1 (unc-51 like autophagy activating kinase 1) protein kinase plays a crucial role in the control of autophagy initiation. This protein is responsible for activating a cascade of signaling pathways which ultimately results in the formation of phagosomes. The phosphorylation of ULK1 by MTORC1 (mammalian target of rapamycin complex) leads to the negative regulation of autophagy. Activation of type I interferon (IFN) receptor (IFNR) results in the activation of ULK1 protein, which, in turn, results in the transcription of gene in a IFN-stimulated response elements (ISRE) and IFNγ activation site (GAS) elements-mediated manner. It is also required for the control of the expression of key IFN-stimulated genes (ISGs).

Physikalische Form

Formulated in 2 5mM Tris-HCl, pH 7.5, 100 mM NaCl, 0.05% Tween-20, 100 μg/ml FLAG peptide, 20% glycerol and 3 mM DTT.

Angaben zur Herstellung

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Rechtliche Hinweise

FreeStyle is a trademark of Invitrogen Corp.

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Die Dokumentenbibliothek aufrufen

Computational analysis of an autophagy/translation switch based on mutual inhibition of MTORC1 and ULK1.
Szymanska P et al
PLoS ONE, 10(3), e0116550-e0116550 (2015)
Deubiquitinase inhibition by WP1130 leads to ULK1 aggregation and blockade of autophagy.
Autophagy, 11(9), 1458-1470 (2015)
Central role of ULK1 in type I interferon signaling.
Saleiro D et al
Cell Reports, 11(4), 605-617 (2015)
Pablo Sánchez-Martín et al.
Journal of cell science, 136(3) (2023-01-17)
Autophagy is a catabolic process during which cytosolic material is enwrapped in a newly formed double-membrane structure called the autophagosome, and subsequently targeted for degradation in the lytic compartment of the cell. The fusion of autophagosomes with the lytic compartment

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