The pH of the media can be 7.0, but depending on the application, it can range from 7.0 to 8.0. The pH can be adjusted using 1N NaOH and 1N HCl.
Wichtige Dokumente
L7275
LB Bouillon
Tablet microbial growth medium
Synonym(e):
Luria Bertani Bouillon
Größe auswählen
CHF 123.00
Voraussichtliches Versanddatum15. April 2025
Größe auswählen
About This Item
CHF 123.00
Voraussichtliches Versanddatum15. April 2025
Empfohlene Produkte
Qualität
for molecular biology
Qualitätsniveau
Sterilität
non-sterile
Form
tablet
Methode(n)
microbiological culture: suitable
Anwendung(en)
food and beverages
microbiology
Lagertemp.
room temp
Eignung
nonselective for Escherichia coli
nonselective for coliforms
Allgemeine Beschreibung
Anwendung
Leistungsmerkmale und Vorteile
- Praktische, kleine Packung macht Wiegen unnötig
- Schnelle Präparation – kein Rühren erforderlich
Komponenten
5 g/l Hefeextrakt
5 g/l NaCl
2,2 g/l inertes Bindemittel
Rekonstituierung
Rechtliche Hinweise
Ähnliches Produkt
Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
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Protokolle
General protocols for growth of competent cells in microbial medium.
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Do the tablets contain any additives to adjust the pH of the media? Also, what should the final pH of the media be?
1 answer-
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Which bacterial culture medium is the best choice for my application?
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Each of the broths will likely grow E. coli very well, but there are still general guidelines for choosing a broth when you are working without a protocol. Generally:LB - Miller and LB - Lennox are used for E. coli growth and maintanence, DNA plasmid production and protein production. The Lennox formulation has a lower salt content required for some salt-sensitive selection antibiotics.LB - Luria low salt is used for special applications where the E. coli growth or other constraints require the lowest possible salt content.Terrific Broth is used for higher yield protein production and high yield DNA plasmid production, because of the faster growth of the E. coli in this medium.SOB is used for protein production, DNA plasmid production and the generation of high-efficiency competent cells.SOC is used for initial growth of competent cells and the transformation procedure.
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What is the difference among the many LB - Lennox products?
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LB - Lennox is available in many types to suit your needs. The different product formats include powder, EZMix™ powder, and tablet form. Each of these forms are available with and without agar for easy LB-agar plate preparation.L3022 (LB Lennox powder)L2897 (LB Lennox powder with agar)L7275 (LB Lennox tablets)L7025 (LB Lennox tablets with agar)L7658 (LB Lennox EZMix(TM) powder)L7533 (LB Lennox EZMix(TM) powder with agar)L5542 (LB Lennox pre-poured agar plates)
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Will adding magnesium to the culture medium increase cell density?
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In microbial broth formulations that do not already contain magnesium, the addition of 10-20 mM MgCl2 or MgSO4 may increase cell densities. You may need to also increase the shaking speed of the incubator.
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What are the differences among the Luria, Lennox and Miller LB formulations?
1 answer-
LB, (originally termed lysogeny broth) was initially composed of tryptone, yeast extract, NaCl and glucose. Soon after, the glucose was omitted (Miller's version), and later the NaCl content lowered by half (Lennox's version). For some applications, even lower salt is required (Luria's low salt version).
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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