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PKH67GL

Sigma-Aldrich

PKH67 grün fluoreszierendes Zelllinker-Kit für die allgemeine Zellmembranmarkierung

Distributed for Phanos Technologies

Synonym(e):

Green PKH membrane labeling kit

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1 KIT
CHF 2’180.00

CHF 2’180.00

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1 KIT
CHF 2’180.00

About This Item

UNSPSC-Code:
12352207
NACRES:
NA.32

CHF 2’180.00

Check Cart for Availability

Qualitätsniveau

200

Verpackung

pkg of 1 kit

Lagerbedingungen

protect from light

Fluoreszenz

λex 490 nm; λem 502 nm (PKH67 dye)

Nachweisverfahren

fluorometric

Versandbedingung

ambient

Lagertemp.

room temp

Verwandte Kategorien

Anwendung

Das grün fluoreszierende Zelllinker-Kit PKH67 für die allgemeine Zellmembranmarkierung wird verwendet:
  • Zur Markierung und weiteren Untersuchung von Exosomen, die von Zellen in dreifach negativem Brustkrebs ausgestoßen wurden.[1]
  • Zur Markierung von apoptotischen Zellen[2], um die Rolle des ανβ5-Rezeptors sowohl bei der Bindung als auch der Internalisierung von apoptotischen Zellen zu untersuchen.[3]
  • In der Fluoreszenzbildgebung.[4]

Dieses Kit ist zur allgemeinen Zellmembranmarkierung vorgesehen. PKH67 hat eine längere aliphatische Kohlenstoffkette als PKH1 und PKH2, zwei weitere grüne Farbstoffe, die zuvor für die In-vitro- und In-vivo-Zellverfolgung beschrieben wurden. Basierend auf der längeren Kette zeigten interne Untersuchungen konsistent einen verringerten Zell-Zell-Transfer für PKH67, verglichen mit PKH2.
Bei In-vivo-Studien mit PKH1 und PKH2 wurde ein langsamer Verlust der Fluoreszenz beobachtet. PKH67 kann ähnliche Eigenschaften aufweisen, da dieses Verhalten charakteristisch für grüne Zelllinker-Farbstoffe zu sein scheint, aber nicht für rote Zelllinker-Farbstoffe. Die Korrelation von der In-vitro-Zellmembranrückhaltung mit der In-vivo-Fluoreszenzhalbwertszeit in sich nicht teilenden Zellen berechnet eine In-vivo-Fluoreszenzhalbwertszeit von 10 bis 12 Tagen für PKH67. Andere grüne Zelllinker-Farbstoffe mit ähnlichen Halbwertszeiten werden verwendet, um den In-vivo-Lymphozyten- und -Makrophagentransport über Zeiträume von 1 bis 2 Monaten zu beobachten, was darauf hindeutet, dass PKH67 auch für In-vivo-Verfolgungsstudien von mittlerer Länge geegígnet sind.

Verlinkung

Zusätzliche technische Informationen zu PKH und CellVue® Zelllinker-Fluoreszenzfarbstoffen einschließlich einer umfassenden Bibliografie finden Sie hier

Rechtliche Hinweise

CellVue is a registered trademark of Phanos Technologies

Nur Kit-Komponenten

Produkt-Nr.
Beschreibung
  • Diluent C 6 x 10
  • PKH67 Cell Linker in ethanol .5 mL

Piktogramme

FlameExclamation mark
GHS02,GHS07

Signalwort

Danger

H-Sätze

H225,H319

Gefahreneinstufungen

Eye Irrit. 2 - Flam. Liq. 2

Lagerklassenschlüssel

3 - Flammable liquids

Flammpunkt (°F)

57.2 °F - closed cup

Flammpunkt (°C)

14 °C - closed cup

Hier finden Sie alle aktuellen Versionen:

Analysenzertifikate (COA)

Lot/Batch Number
MKCV4102
MKCQ9079
MKCP3216
MKCQ0194
MKCK9600

Die passende Version wird nicht angezeigt?

Wenn Sie eine bestimmte Version benötigen, können Sie anhand der Lot- oder Chargennummer nach einem spezifischen Zertifikat suchen.

Suche nach COA

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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Exosomes from triple-negative breast cancer cells can transfer phenotypic traits representing their cells of origin to secondary cells
O Brien K, et al.
European Journal of Cancer, 49(8), 1845-1859 (2013)
Anjali P Kusumbe et al.
Stem cells (Dayton, Ohio), 27(3), 498-508 (2009-03-04)
Recruitment and localization of endothelial precursors within tumors is a potential area for the development of therapeutics, because their functional contribution to tumor vasculature is realized to be important for cancer cell survival. However, the exact nature of the recruited
Nobuyoshi Kosaka et al.
The Journal of biological chemistry, 287(2), 1397-1405 (2011-11-30)
Normal epithelial cells regulate the secretion of autocrine and paracrine factors that prevent aberrant growth of neighboring cells, leading to healthy development and normal metabolism. One reason for tumor initiation is considered to be a failure of this homeostatic cell
Hybrid microscaffold-based 3D bioprinting of multi-cellular constructs with high compressive strength: A new biofabrication strategy
Yu Jun T, et al.
Scientific Reports, 6, 39140-39140 (2016)
Dandan Ma et al.
Cells, 9(4) (2020-04-01)
Human salivary histatin 1 (Hst1) and Hst2 exhibit a series of cell-activating properties (e.g., promoting adhesion, spreading, migration and metabolic activity of mammalian cells). In contrast, Hst5 shows an anti-fungal property but no cell-activating properties. Previous findings suggest that their
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Questions

1–10 of 12 Questions  

  1. I mark mitochondria for injection with PKH26 Red Fluorescent Cell Linker Kit for General Cell Membrane Labeling. I want to use another dye to mark another type of mitochondria and inject them together in the same fish egg. Is PKH67 ok for this like PKH26?

    1 answer

    1. Yes, Product PKH67 would be suitable for this application. In this paper, both Products PKH26 and PKH67 are used in a similar application:
      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5679712/

      Helpful?

  2. What can be the alternative for removing excess dye after labeling of exosomes using PKH67 dye apart from ultracentrifugation?

    1 answer

    1. Excess dye is bound to serum or albumin which is added to the cell/dye mixture as a stop reaction. The recommended clean up or dye removal is by standard centrifugation (400 x g for 10 minutes, wash, and repeat). See the Product Information Sheet, page 3, bullet point 8 for more information:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/984/984/pkh67glbul.pdf

      Alternate methods for excess dye removal have not been investigated.

      Helpful?

  3. Can we remove the excess PKH67 DYE used for the labeling of exosomes using exosome spin column rather than ultracentrifugation.

    1 answer

    1. The recommended exosome labeling protocol has been optimized to assure the best possible results. The use of exosome spin columns with the PKH and CellVue products has not been validated. See below for a link to our exosome labeling protocol. A spin column method may not offer the best results.

      https://www.sigmaaldrich.com/technical-documents/protocol/cell-culture-and-cell-culture-analysis/imaging-analysis-and-live-cell-imaging/exosome-labeling-pkh

      Helpful?

  4. Will Product PKH67, Green Fluorescent Cell Linker Kit, stain dead cells?

    1 answer

    1. As long as the cell has an intact membrane, the PKH76 dye can label the cell.

      Helpful?

  5. What is the difference between Green Fluorescent Cell Linker Kits PKH2 and PKH67?

    1 answer

    1. PKH2 was one of the early PKH dyes.  The PKH67 dye has a longer aliphatic tail.  There is reduced cell-celldye transfer for PKH67 as compared with PKH2.

      Helpful?

  6. What method of fixation can be used for tissue/cells with the PKH Fluorescent Cell Linker Kit for General Cell Membrane Labeling dyes?

    1 answer

    1. A protocol for visualization of tissue sections can be found in the technical bulletin.  For visualization of stained cells by immunofluorescence or flow cytometry, the cells can be fixed in 2% paraformaldehyde for 15 minutes. The use of other organic solvents will extract the dye from the cells.  If internal labeling is desired, the cells can be permeabilized with saponin (50-75 μg/mL). Here is the link to the Troubleshooting Guide.

      Helpful?

  7. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  8. What is the excitation and emission spectra for Product PKH67GL, Green Fluorescent Cell Linker Kit?

    1 answer

    1. The spectra can be found on the product insert.The product has a maximum excitation at 490 nm and maximum emission at 502 nm.

      Helpful?

  9. How many cells can be stained with Product PKH67GL, Green Fluorescent Cell Linker Kit?

    1 answer

    1. This kit can stain 50 × 107 cells if used as directed (1 × 107 cells stained with 2 × 10-6 M PKH67).

      Helpful?

  10. When using Product PKH67GL, PKH67 Green Fluorescent Cell Linker Kit for General Cell Membrane Labeling, how long will the cells remain stained after treatment?

    1 answer

    1. Labeled cells that have been washed can be visualized in culture up to 100 days after staining (for non-dividing cells). The dye itself is stable and will divide equally when the cells divide. After staining with PKH dyes, you can observe as many as 8 divisions depending on how brightly the cells were stained initially and the amount of surface area on the cells. Most commonly, 4-6 divisions can be visualized.

      Helpful?

1–10 of 12 Questions  

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