P5899

Poly-ʟ-Lysine Hydrobromide

synthetic, mol wt ≥300,000, powder, γ-irradiated, suitable for cell culture, BioXtra

• Synonym(e): PDL HBr
• CAS-Nummer: 25988-63-0
• MDL-Nummer: MFCD00237230
• UNSPSC-Code: 12352202
• eCl@ss: 32160406
• PubChem Substanz-ID: 24898718
• NACRES: NA.75

Eigenschaften

• Produktbezeichnung: Poly-L-Lysin -hydrobromid, mol wt ≥300,000, lyophilized powder, γ-irradiated, BioXtra, suitable for cell culture
• Biologische Quelle: synthetic (organic)
• Qualitätsniveau: 200
• Sterilität: γ-irradiated
• Produktlinie: BioXtra
• Form: lyophilized powder
• Mol-Gew.: ≥300,000
• Verpackung: pkg of 5 mg
• Methode(n): cell culture | mammalian: suitable
• Oberflächendeckung: 4 μg/cm²
• Verunreinigungen: <10% water (by Karl Fischer)
• Löslichkeit: H₂O: soluble 50 mg/mL, clear to very slightly hazy, colorless to faintly yellow
• Versandbedingung: ambient
• Lagertemp.: −20°C
• SMILES String: Cl.NCCCCC(N)C(O)=O
• InChI: 1S/C18H38N6O4/c19-10-4-1-7-13(22)16(25)23-14(8-2-5-11-20)17(26)24-15(18(27)28)9-3-6-12-21/h13-15H,1-12,19-22H2,(H,23,25)(H,24,26)(H,27,28)/t13-,14-,15-/m0/s1
• InChIKey: WBSCNDJQPKSPII-KKUMJFAQSA-N

Beschreibung

Anwendung

Poly-L-lysine hydrobromide has been used to coat:

• 2-well plates before seeding muscle cells in a research study on rainbow trout myotube formation[1]
• flat bottom 96-well plates for in vitro neuronal stem cell differentiation[2]
• T75 flasks for primary astrocytic cultures[3]

Biochem./physiol. Wirkung

Poly-L-lysine serves as a coating substance to ensure better attachment of cells to culture surfaces.[4] Research shows that plates coated with poly-L-lysine (PLL) demonstrated an accelerated cell growth rate.[5] Culturing mesenchymal stem cells (MSCs) on PLL-coated substrates has been found to safeguard the stemness properties of these cells and inhibit senescence. PLL-coated plates have demonstrated enhanced MSC growth and up-regulated the expression of genes associated with cell adhesion, differentiation, proliferation, and signaling. Moreover, porous PLL-coated surfaces have shown improvements in cell proliferation and the osteogenic differentiation of MSCs.[5]

Komponenten

Poly-L-Lysin ist ein positiv geladenes Aminosäurepolymer mit etwa einem HBr pro Lysin-Rest. Das Hydrobromid ermöglicht die kristalline, wasserlösliche Form des Poly-L-Lysins. Eine geringe Produktmenge kann in der ß-Struktur zu finden sein, da das HBr die Wasserstoffbindung zwischen Aminogruppen und den Carboxylgruppen oder N oder O enthaltenden Gruppen beeinträchtigt.

Vorsicht

Sterile Lösungen sind bei Lagerung zwischen 2 und 8 °C bis zu 2 Jahre haltbar. Das Lyophilisat sollte bei -20 °C aufbewahrt werden.

Hinweis zur Analyse

Molecular weight based on viscosity. Also assayed by MALLS.

Sonstige Hinweise

For additional technical information on polyamino acids please visit the Polyamino acid FAQ resource.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)