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MAK122

Sigma-Aldrich

Phospholipid-Assaykit

sufficient for 100 colorimetric or fluorometric tests

Synonym(e):

Phospholipid-Quantifizierungskit

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1 KIT
CHF 571.00

CHF 571.00

Voraussichtliches Versanddatum02. Juni 2025

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1 KIT
CHF 571.00

About This Item

UNSPSC-Code:
12161503
NACRES:
NA.84

CHF 571.00

Voraussichtliches Versanddatum02. Juni 2025

Bulk-Bestellung anfordern

Verwendung

sufficient for 100 colorimetric or fluorometric tests

Anwendung(en)

cosmetics
food and beverages
pharmaceutical

Nachweisverfahren

colorimetric
fluorometric

Versandbedingung

dry ice

Lagertemp.

−20°C

Verwandte Kategorien

Allgemeine Beschreibung

Phospholipide sind eine Lipidklasse; sie sind Hauptbestandteile von Zellmembranen und spielen eine wichtige Rolle für die Signalübertragung. Die meisten Phospholipide enthalten ein Diglyzerid, eine Phosphatgruppe und ein Cholin.

In diesem Assay werden Phospholipide (etwa Lecithin, Lysolecithin und Sphingomyelin) enzymatisch zu Cholin hydrolysiert, was mithilfe von Cholinoxidase und einem H2O2-spezifischen Farbstoff festgestellt wird. Dies ergibt ein kolorimetrisches (570 nm) bzw. fluorometrisches (λex = 530/λem = 585 nm) Produkt, das direkt proportional zur Phospholipidkonzentration in der Probe ist. Der lineare Detektionsbereich liegt bei 3–200 μM für kolorimetrische Assays und bei 0,6–20 μM für fluorometrische Assays.

Anwendung

Das Phospholipid-Assaykit wird in der biochemischen Analyse[1] und zur Quantifizierung von Phospholipiden verwendet.[2][3][4][5]

Leistungsmerkmale und Vorteile

Kompatibel mit Handhabungssystemen für hohen Durchsatz.

Eignung

Geeignet für den Nachweis von Phospholipiden in biologischen Proben

Prinzip

In diesem Assay werden Phospholipide (etwa Lecithin, Lysolecithin und Sphingomyelin) enzymatisch zu Cholin hydrolysiert, was mithilfe von Cholinoxidase und einem H2O2-spezifischen Farbstoff festgestellt wird. Dies ergibt ein kolorimetrisches (570 nm) bzw. fluorometrisches (λex = 530/λem = 585 nm) Produkt, das direkt proportional zur Phospholipidkonzentration in der Probe ist. Der lineare Detektionsbereich liegt bei 3–200 μM für kolorimetrische Assays und bei 0,6–20 μM für fluorometrische Assays.

Piktogramme

Health hazard
GHS08

Signalwort

Danger

H-Sätze

H334,H412

Gefahreneinstufungen

Aquatic Chronic 3 - Resp. Sens. 1

Lagerklassenschlüssel

10 - Combustible liquids

Hier finden Sie alle aktuellen Versionen:

Analysenzertifikate (COA)

Lot/Batch Number
122CE11A20
122CE11A20
122CE08A29
122CE08A29
122CD11A30

Die passende Version wird nicht angezeigt?

Wenn Sie eine bestimmte Version benötigen, können Sie anhand der Lot- oder Chargennummer nach einem spezifischen Zertifikat suchen.

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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Inhibiting mevalonate pathway enzymes increases stromal cell resilience to a cholesterol-dependent cytolysin.
Griffin S, et al.
Scientific Reports, 7(1), 17050-17050 (2017)
Vineela Parvathaneni et al.
Pharmaceutics, 12(3) (2020-03-04)
Non-small cell lung cancer (NSCLC) is a global disorder, treatment options for which remain limited with resistance development by cancer cells and off-target events being major roadblocks for current therapies. The discovery of new drug molecules remains time-consuming, expensive, and
Hypoxia-inducible factor-1 alpha as a therapeutic target for primary effusion lymphoma.
Shrestha P, et al.
PLoS Pathogens, 13(9), e1006628-e1006628 (2017)
Kevin M Tharp et al.
American journal of physiology. Gastrointestinal and liver physiology, 310(10), G855-G864 (2016-04-02)
Gallstone disease is a widespread disorder costing billions for annual treatment in the United States. The primary mechanisms underlying gallstone formation are biliary cholesterol supersaturation and gallbladder hypomotility. The relative contribution of these two processes has been difficult to dissect
Renoprotective effect of curcumin against the combined oxidative stress of diabetes and nicotine in rats.
Ibrahim ZS, et al.
Molecular Medicine Reports, 13(4), 3017-3026 (2016)
Alle zugehörigen Dokumente anzeigen

Questions

1–5 of 5 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Helpful?

  3. Is the 10 ml of Assay Buffer in MAK122 sufficient for 100 assays? Also, what amount of sample/buffer should be used?

    1 answer

    1. 1. The general recommendation for homogenization is a 1:10 solid to buffer ratio, for example, 20mg solid with 200 uL of Buffer. However, this ratio may not always be optimal. If there are concerns about having enough assay buffer for both homogenization and assays, the homogenization can be run with a different homogenization buffer, provided that it contains at least 0.5% Triton X-100. Please note that the amount of Assay Buffer needed per sample should be enough to yield 40 microliters of supernatant, unless you choose to use your own homogenization buffer with 0.5% Triton X-100.

      2. The kit is usually tested on tissue samples. However, it has not been tested on tissues themselves.

      3. For homogenizing samples, they can be run through 10-20 passes in a Dounce homogenizer on ice or by sonication, preferably performed in an ice-water bath. The degree of tissue lysis can be checked under a microscope. After homogenization, it is recommended to centrifuge the homogenate at 14,000 g for 10 minutes.

      Helpful?

  4. Why is cold assay buffer listed as a potential cause for the assay not working correctly? Can a 37 or 25 degree water bath be used to thaw the assay buffer? Can the assay buffer be stored at room temperature instead of at -20 degrees?

    1 answer

    1. The troubleshooting bulletin for MAK122 suggests that if the assay does not work, it could be due to the assay buffer not being at room temperature. This is because the enzyme is more active at room temperature than in colder conditions. Although stability data for the assay buffer at room temperature is not available, it is recommended to store it at -20 degrees or evaluate the suitability of room temperature storage. While the composition of the assay buffer is proprietary, it is expected to be stable at room temperature. Additionally, a 37 or 25 degree C water bath can be used to thaw the assay buffer, with a caution against excessive heating and a recommendation to remove it from the water bath once it reaches room temperature.

      Helpful?

  5. To prepare the 0.5% Triton X-100 solution to dilute my samples, should I dilute Triton x-100 in water, in the assay buffer, or something else?

    1 answer

    1. The 0.5% Triton X-100 solution to be used as the sample diluent may be prepared using water.

      Helpful?

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