K0879

Kanamycin -sulfat aus Streptomyces kanamyceticus

powder, γ-irradiated

• Synonym(e): Kanamycin A
• Empirische Formel (Hill-System): C₁₈H₃₆N₄O₁₁ · H₂O₄S
• CAS-Nummer: 25389-94-0
• Molekulargewicht: 582.58
• EG-Nummer: 246-933-9
• MDL-Nummer: MFCD00070253
• UNSPSC-Code: 12352200
• PubChem Substanz-ID: 57654313
• NACRES: NA.85

Eigenschaften

• Biologische Quelle: Streptomyces kanamyceticus
• Qualität: for molecular biology
• Sterilität: γ-irradiated
• Form: powder
• Wirksamkeit: ≥700 μg per mg
• Farbe: white to off-white
• pH-Wert: 6.5-8.5(1% solution)
• Wirkungsspektrum von Antibiotika: Gram-negative bacteria; Gram-positive bacteria; mycoplasma
• Wirkungsweise: protein synthesis | interferes
• Lagertemp.: 2-8°C
• Eignung: nonselective for Escherichia coli; nonselective for coliforms
• SMILES String: OS(O)(=O)=O.NC[C@H]1O[C@H](O[C@@H]2[C@@H](N)C[C@@H](N)[C@H](O[C@H]3O[C@H](CO)[C@@H](O)[C@H](N)[C@H]3O)[C@H]2O)[C@H](O)[C@@H](O)[C@@H]1O
• InChI: 1S/C18H36N4O11.H2O4S/c19-2-6-10(25)12(27)13(28)18(30-6)33-16-5(21)1-4(20)15(14(16)29)32-17-11(26)8(22)9(24)7(3-23)31-17;1-5(2,3)4/h4-18,23-29H,1-3,19-22H2;(H2,1,2,3,4)/t4-,5+,6-,7-,8+,9-,10-,11-,12+,13-,14-,15+,16-,17-,18-;/m1./s1
• InChIKey: OOYGSFOGFJDDHP-KMCOLRRFSA-N

Beschreibung

Allgemeine Beschreibung

Chemical structure: aminoglycoside

Kanamycin, A from Streptomyces kanamyceticus is a polycationic aminoglycoside and a broad-spectrum antibiotic. Structurally, it comprises hydroxyl and amino functional groups.

Anwendung

Kanamycin sulfate Streptomyces kanamyceticus has been used as an antibiotic component in Murashige and Skoog medium for the selection of tobacco tissue culture explants. It has also been used as an antibiotic in Luria broth for culturing Escherichia coli K-12 strain MG1655 cells for secretion assay and E. coli TV1061 cells for immobilization studies.

Biochem./physiol. Wirkung

Kanamycin′s hydroxyl and amino functional groups are crucial for its membrane action functionality. However, it also exhibits toxicity towards mammalian cells. It prevents contamination in cell culture as well as a selective agent for bacteria with kanamycin resistance.

Wirkungsweise: Das Produkt wirkt, indem es an die 70S-Ribosomen-Untereinheit bindet und so die Translokation und Auslösung von Fehlkodierungen hemmt.

Widerstandsweise: Aminoglycosid-modifizierende Enzyme (einschließlich Acetyltransferase, Phosphotransferase, Nukleotidyltransferase) können dieses Antibiotikum verändern und dessen Wechselwirkung mit Ribosomen verhindern.

Antimikrobielles Spektrum: Kanamycinsulfat ist wirksam gegen gramnegative und grampositive Bakterien und Mycoplasma.

Prinzip

Kanamycin binds to the 30S ribosomal subunit and inhibits protein synthesis, leading to cell death.

Angaben zur Herstellung

Prepare stock solution directly in the vial with sterile water to a final concentration of 10mg/mL. Stock solution should be stored at 2-8°C.

Sicherheitshinweise

• Piktogramme: GHS08
• Signalwort: Danger
• H-Sätze: H360D
• P-Sätze: P201 - P308 + P313
• Gefahreneinstufungen: Repr. 1B
• Lagerklassenschlüssel: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 2
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)