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Merck

F6136

Sigma-Aldrich

Anti-Horse Spleen Ferritin antibody produced in rabbit

affinity isolated antibody, lyophilized powder

Synonym(e):

Ferritin Antibody

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About This Item

MDL-Nummer:
UNSPSC-Code:
12352203
NACRES:
NA.46

Biologische Quelle

rabbit

Konjugat

unconjugated

Antikörperform

affinity isolated antibody

Antikörper-Produkttyp

primary antibodies

Klon

polyclonal

Form

lyophilized powder

Speziesreaktivität

horse

Methode(n)

immunoelectrophoresis: suitable
immunoelectrophoresis: suitable
indirect ELISA: 1:10,000

Lagertemp.

2-8°C

Posttranslationale Modifikation Target

unmodified

Angaben zum Gen

horse ... Ftl(100051593)

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Allgemeine Beschreibung

Ferritin consists of homogenous protein segment called as apoprotein and an iron segment which binds to the apoprotein. It is composed of heavy (H) and light (L) chains.
Ferritin is the storage form of iron in mammals, found mainly in liver, bone marrow and spleen. It is also present in small intestine, placenta, kidney and heart. Horse spleen ferritin is made up of 24 identical subunits. Ferritin of different organs is distinct with different electrophoretic mobility and possible functional differences.
.

Spezifität

The antibody specifically recognizes ferritin from horse spleen.

Immunogen

Purified horse spleen ferritin

Anwendung

Anti-Horse Spleen Ferritin antibody was used for immunocytochemistry of rat brain sections at a working antibody dilution of 1:2000 and in mouse brain sections at 1:1000. The antibody was used for immunoblotting using cell lysates of mouse primary erythroblasts and human ovarian carcinoma cells and human bronchial epithelial cells at a working dilution of 1:1000. The antibody is suitable for immunoelectrophoresis.
Horse Spleen Ferritin antibody has been used:
  • immunofluorescence
  • in western blotting
  • in immunohistochemistry

Biochem./physiol. Wirkung

Ferritin iron is oxidized from Fe(II) to Fe(III) in the presence of the enzyme ferroxidase.

Physikalische Form

Lyophilized from 0.01 M sodium phosphate, 0.015 M sodium chloride, pH 7.2

Rekonstituierung

Reconstitute with 0.135 M sodium chloride.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 2

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)


Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Die Dokumentenbibliothek aufrufen

Jan A Gorter et al.
Epilepsia, 46(9), 1371-1379 (2005-09-09)
Iron accumulation in the brain has been associated with neurodegenerative disorders, including epilepsy. In our previous SAGE study, we showed that ferritin, an iron-storage protein, was one of the genes (Ferritin-H) that showed overexpression before the chronic epileptic phase. In
New functions for an iron storage protein: the role of ferritin in immunity and autoimmunity
Recalcati S, et al.
Journal of Autoimmunity, 30(1-2), 84-89 (2008)
Microglial pathology in Down syndrome
Xue Q S and Streit W J
Acta Neuropathologica, 122(4), 455-455 (2011)
Biocompatibility of ferritin-based nanoparticles as targeted MRI contrast agents
Charlton J R, et al.
Nanomedicine: Nanotechnology, Biology, and Medicine, 12(6), 1735-1745 (2016)
Structure and physiological functions of ferritin
Granick, S
Physiological Reviews, 31(4), 489-511 (1951)

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