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I7633

Sigma-Aldrich

IMDM

With ʟ-glutamine and HEPES, without sodium bicarbonate, powder, suitable for cell culture

Synonym(e):

IMDM

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About This Item

MDL-Nummer:
MFCD00217469
UNSPSC-Code:
12352207
NACRES:
NA.75

product name

Modifiziertes Dulbecco-Medium nach Iscove, With L-glutamine and 25 mM HEPES, without sodium bicarbonate, powder, suitable for cell culture

Form

powder

Methode(n)

cell culture | hybridoma: suitable
cell culture | mammalian: suitable

Komponenten

phenol red: yes
glucose: yes
L-glutamine: yes

Versandbedingung

ambient

Lagertemp.

2-8°C

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Allgemeine Beschreibung

Iscove′s Modified Dulbecco′s medium is a modification of Dulbecco′s Modified Eagle′s Medium (DME) containing selenium, additional amino acids, and vitamins, sodium pyruvate, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer, and potassium nitrate instead of ferric nitrate. Iscove′s medium supports murine B lymphocytes, hemopoietic tissue from bone marrow, B cells stimulated with lipopolysaccharide, T lymphocytes, and a variety of hybrid cells.

Anwendung

Iscove′s Modified Dulbecco′s Medium has been used to culture KG1a cells (human leukemic cell line) and Chinese hamster ovary (CHO) DG44 cell line.
Reported to support murine B lymphocytes, hematopoietic tissue from bone marrow, B cells stimulated with lipopolysaccharide, T lymphocytes, and a variety of hybrid cells.

Menge

Formulated to contain 17.7 grams of powder per liter of medium.

Rekonstituierung

Supplement with 3.024 g/L sodium bicarbonate.

Ergänzung

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Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 2

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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