This product is NOT tested for injection into animals. The product is NOT sterile or tested for endotoxin levels.
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H3506
Hyaluronidase aus Rindertestes
Type I-S, lyophilized powder, 400-1000 units/mg solid
Synonym(e):
Hyaluronat-4-glycanhydrolase, Hyaluronoglucosaminidase
Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise
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100 MG
CHF 63.40
500 MG
CHF 189.00
1 G
CHF 329.00
5 G
CHF 1’260.00
Alle Fotos(5)
Größe auswählen
Ansicht ändern
100 MG
CHF 63.40
500 MG
CHF 189.00
1 G
CHF 329.00
5 G
CHF 1’260.00
About This Item
CAS-Nummer:
EG-Nummer:
MDL-Nummer:
UNSPSC-Code:
12352204
NACRES:
NA.54
Empfohlene Produkte
Typ
Type I-S
Qualitätsniveau
Form
lyophilized powder
Spezifische Aktivität
400-1000 units/mg solid
Mol-Gew.
~55 kDa (four subunits of 14 kDa each)
Methode(n)
cell culture | mammalian: suitable
single cell analysis: suitable
Anwendung(en)
diagnostic assay manufacturing
Versandbedingung
wet ice
Lagertemp.
−20°C
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Anwendung
Biochem./physiol. Wirkung
Diese Enzyme spalten randomisiert β-N-acetylhexosamin-[1→4]-glycosidische Bindungen in Hyaluronsäure, Chondroitin und Chondroitin-Sulfaten.
Hyaluronidase baut Hyaluronan ab und wird während der Krebsprogredienz unangemessen reguliert.
Einheitendefinition
Eine Einheit baut 0,75 μg des Polysaccharids Hyaluronsäure pro Minute bei einem pH-Wert von 5,35 und einer Temperatur von 37 ºC ab (bestimmt durch turbidimetrische Messung der Absorption (λ: 600 nm), wenn sie nach 45 Minuten mit BSA komplexiert wird).
Sonstige Hinweise
Weitere Informationen über Enzyme für die Analyse komplexer Kohlenhydrate finden Sie unter www.sigma-aldrich.com/enzymeexplorer
Empfehlung
Produkt-Nr.
Beschreibung
Preisangaben
Signalwort
Danger
H-Sätze
P-Sätze
Gefahreneinstufungen
Resp. Sens. 1
Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Persönliche Schutzausrüstung
Eyeshields, Gloves, type N95 (US)
Hier finden Sie alle aktuellen Versionen:
Analysenzertifikate (COA)
Lot/Batch Number
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Protokolle
To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.
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Can this be used in animal models in vivo?
1 answer-
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Is it acceptable to use H3506 SLCH0779 directly in synovial fluid without dilution?
1 answer-
It is generally not a standard practice to simply sprinkle hyaluronidase (such as H3506 SLCH0779) directly into synovial fluid. For a more formal and generally recognized method, a 0.05% solution in phosphate buffer is recommended, with one drop added per milliliter of fluid. This allows for the clarification of synovial fluids to make cell counts possible. Therefore, directly using H3506 SLCH0779 in synovial fluid without dilution may not be the most common practice.
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Is there any information available regarding whether hyaluronidase impacts body fluid crystals?
1 answer-
Hyaluronidase is not expected to impact crystals. It is specific to hyaluronan or Hyaluronic acid and may be used with breast and possibly other tissues. However, crystals are typically inorganic salts, with body fluid crystals typically being pyrophosphate. Therefore, hyaluronidase should have no effect upon pyrophosphate crystals.
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What does the type refer to i.e. type I-S?
1 answer-
The type designations for the hyaluronidase enzyme products offered are in-house designations and do not represent any kind of biochemical significance. The types can refer to the order of addition of the product to the catalog, source, physical form, activity, tests performed, etc.
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Can I dissolve H3506 in DMEM/F12 media or PBS before use for tissue dissociation?
1 answer-
It is recommended to dissolve this product at 1 mg/mL in 0.02 M phosphate buffer, pH 7, containing 77 mM sodium chloride and 0.01% BSA. Solubilizing directly into the medium has not been tested, but may be suitable. It is important to include BSA to ensure stability.
See the link below for the recommended instructions:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/296/016/h3506-pis-ms.pdfHelpful?
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I am looking for specific inhibitor for extrasynaptic GABA Rs. The one I found in Sugma is Gaboxadol hydrochloride for ɣ containing containing. Which is the best inhibitor do you have. Is also any extrasynaptic GABA inhibitors for ALPHA subunits GABA?
1 answer-
Searching via the Gene Lookup tool on the main webpage search bar can provide a product list of various related products for any gene product, including agonists, antagonists, antibodies, proteins, RNAi and related gene editing products. Go to the main search bar, select "Genes" from the left dropdown and enter the gene name, gene symbol or gene id to pull a list of gene candidates. Next, select the appropriate gene by clicking on it - the related products will display. For example here is the list of gene products for the GABA A receptor alpha one (GABRA1) https://www.sigmaaldrich.com/US/en/genes/gabra1
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What is the shelf life of Product H3506, Hyaluronidase from bovine testes?
1 answer-
Product H3506, Hyaluronidase from bovine testes, has a Recommended Retest period of two years (period of time between the Quality Control Release and Recommended Retest dates). This date assigns the time frame during which the batch is expected to remain within established specifications if stored under Sigma-Aldrich’s defined conditions, which for product H3506 is at -20°C. This information is provided on the Product's lot specific Certificate of Analysis.
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How is a solution of Hyaluronidase from bovine testes, Product H3506, stored?
1 answer-
Prepare fresh solutions directly before use since stock solutions are not stable for storage.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Can Hyaluronidase from bovine testes, Product H3506, be used on humans?
1 answer-
No. None of our packaged research products are for human use.
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