The solubility of H375, Hyaluronidase from bovine testes, is 100 mg/mL in water. To achieve a higher solubility, it is advised to warm the tube at 37°C and shake it in an ultrasonic bath for a period. The stock solution can be stored at temperatures below -20°C for several months.
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H3757
Hyaluronidase aus Rindertestes
Type VIII, lyophilized powder, 300-1,000 U/mg
Synonym(e):
Hyaluronat-4-glycanhydrolase, Hyaluronoglucosaminidase
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100 MG
CHF 108.00
10 X 100 MG
CHF 833.00
CHF 108.00
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Größe auswählen
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100 MG
CHF 108.00
10 X 100 MG
CHF 833.00
About This Item
CAS-Nummer:
EG-Nummer:
MDL-Nummer:
UNSPSC-Code:
12352204
NACRES:
NA.54
CHF 108.00
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Empfohlene Produkte
Biologische Quelle
bovine testis
Qualitätsniveau
Konjugat
conjugate (Glucosaminoglycan)
Typ
Type VIII
Form
lyophilized powder
Spezifische Aktivität
300-1,000 U/mg
Mol-Gew.
~55 kDa (four subunits of 14 kDa each)
Relevante Krankheit(en)
cancer
Lagertemp.
−20°C
Angaben zum Gen
cow ... HYAL1(515397) , HYAL2(281838)
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Verwandte Kategorien
Biochem./physiol. Wirkung
Diese Enzyme spalten randomisiert β-N-acetylhexosamin-[1→4]-glycosidische Bindungen in Hyaluronsäure, Chondroitin und Chondroitin-Sulfaten.
Hyaluronidase baut Hyaluronan ab und wird während der Krebsprogredienz unangemessen reguliert.
Einheitendefinition
One unit will degrade 0.75 μg of the polysaccharide hyaluronic acid per minute at pH 5.35 at 37 ºC (as measured by turbidimetric absorbance (λ: 600nm) when complexed with BSA after 45 minutes).
Angaben zur Herstellung
Prepared from aseptic filled solution of Type I-S.
Sonstige Hinweise
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
Signalwort
Danger
H-Sätze
P-Sätze
Gefahreneinstufungen
Resp. Sens. 1
Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Persönliche Schutzausrüstung
Eyeshields, Gloves, type N95 (US)
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Artikel
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Protokolle
To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.
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What is the recommended method for reconstituting item H375, Hyaluronidase from bovine testes?
1 answer-
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Good Morning, I our lab we got this product (H3757) and we would like to know how to obtain the package insert since we are not sure how to store it or after open expiration date. Not lot number on the bottle so not sure how to look for it. Thank you
1 answer-
Hyaluronidase from bovine testes may be reconstituted at 1 mg/mL in 0.02 M sodium phosphate buffer, pH 7.0 containing 77mM sodium chloride and 0.01% bovine serum albumin. For more information please view the assay below:
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How is product H3757, Hyaluronidase from bovine testes, solubilized?
1 answer-
Product H3757, Hyaluronidase from bovine testes, may be reconstituted at 1 mg/mL in 0.02 M sodium phosphate buffer, pH 7.0 containing 77mM sodium chloride and 0.01% bovine serum albumin.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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