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G6048
Galactokinase human
recombinant, expressed in E. coli
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200 μG
CHF 621.00
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Größe auswählen
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200 μG
CHF 621.00
About This Item
Empfohlene Produkte
Rekombinant
expressed in E. coli
Qualitätsniveau
Form
solution
Spezifische Aktivität
≥1400 unit/μg protein
Mol-Gew.
42 kDa
Versandbedingung
dry ice
Lagertemp.
−70°C
Verwandte Kategorien
Biochem./physiol. Wirkung
Galactokinase catalyzes the phosphorylation of αD-galactose to produce galactose-1-phosphate as part of the Leloir pathway[1].
Physikalische Eigenschaften
N-terminal GST-tagged 42 kDa full length protein
Einheitendefinition
One unit will convert 1.0 picomole of galactose to galactose-1-phosphate per minute at pH 7.4 at 30 °C.
Physikalische Form
Supplied as a solution in 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, 3 mM DTT and 10-250 mM imidazole.
Signalwort
Danger
H-Sätze
Gefahreneinstufungen
Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
Lagerklassenschlüssel
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
WGK
WGK 1
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
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Shivani Malik et al.
Nucleic acids research, 40(8), 3348-3363 (2011-12-27)
Recently, we have demonstrated a predominant association of Rad26p with the coding sequences but not promoters of several GAL genes following transcriptional induction. Here, we show that the occupancy of histone H2A-H2B dimer at the coding sequences of these genes
Dariusz Abramczyk et al.
Eukaryotic cell, 11(3), 334-342 (2012-01-03)
The regulation of the Saccharomyces cerevisiae GAL genes in response to galactose as a source of carbon has served as a paradigm for eukaryotic transcriptional control over the last 50 years. Three proteins--a transcriptional activator (Gal4p), an inhibitor (Gal80p), and
Gan Dai et al.
Journal of virological methods, 182(1-2), 18-26 (2012-03-13)
Bacterial artificial chromosome (BAC) recombineering using galK selection allows DNA cloned in Escherichia coli to be modified without introducing an unwanted selectable marker at the modification site. Genomes of some herpesviruses have a pair of inverted repeat sequences that makes
Sarah Piccirillo et al.
BMC biotechnology, 11, 120-120 (2011-12-07)
Precise targeted mutations are defined as targeted mutations that do not require the retention of other genetic changes, such as marker genes, near the mutation site. In the yeast, S. cerevisiae, there are several methods for introducing precise targeted mutations
Bridget L Baumgartner et al.
Proceedings of the National Academy of Sciences of the United States of America, 108(52), 21087-21092 (2011-12-14)
Cells have evolved complex regulatory networks that reorganize gene expression patterns in response to changing environmental conditions. These changes often involve redundant mechanisms that affect various levels of gene expression. Here, we examine the consequences of enhanced mRNA degradation in
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