The product is offered on the basis of activity. The purity has not been determined.
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C1184
Cellulase aus Aspergillus niger
powder, ≥0.3 units/mg solid
Synonym(e):
1,4-(1,3:1,4)-β-D-Glucan-4-glucanohydrolase
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CHF 134.00
100000 UNITS
CHF 423.00
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5000 UNITS
CHF 60.00
25000 UNITS
CHF 134.00
100000 UNITS
CHF 423.00
About This Item
Empfohlene Produkte
Form
powder
Qualitätsniveau
Spezifische Aktivität
≥0.3 units/mg solid
Grünere Alternativprodukt-Eigenschaften
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
Grünere Alternativprodukt-Kategorie
, Enabling
Lagertemp.
2-8°C
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Allgemeine Beschreibung
The FDA recognizes cellulase from A. niger as GRAS (Generally Regarded As Safe) if non-pathogenic and non-toxigenic strains, as well as current good manufacturing practices, are used in production.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been used as enzyme for alternative energy. For more information see the Enzymes for Alternative Energy Research.
Cellulase belongs to the family of glycoside hydrolase,[1] which is secreted by various cellulolytic microorganisms.[2]
Cellulase belongs to the family of glycoside hydrolase,[1] which is secreted by various cellulolytic microorganisms.[2]
Anwendung
Cellulase from Sigma has been used to study the ability of several of its possible substrates, cellulose, Avicel PH-101, and filter paper, to protect enzyme activity during monogastric diegstion in animal and avian digestive tracts.[3]
The enzyme has also been approved as a secondary direct food additive as an aid in clam and shrimp processing.[4]
Biochem./physiol. Wirkung
Cellulase aus Aspergillus niger katalysiert die Hydrolyse von Endo-1,4-β-D-glycosidischen Verbindungen in Zellulose, Lizenin, Gerstenglucan und die Cellooligosacchariden-Cellotriose zu Cellohexaose. Bewirkt keine Spaltung von Cellobiose oder p-Nitrophenyl-β-D-glucosid. Dieses Enzym spaltet auch intaktes Glykosaminoglykan aus einem Kernpeptid durch Hydrolyse der Xylosylserin-Verbindung.
Einheitendefinition
1 U setzt 1.0 μmol Glucose aus Cellulose pro Stunde frei bei pH 5.0 und 37 °C (2 Std Inkubationszeit).
Sonstige Hinweise
Weitere Informationen über Enzyme für die Analyse komplexer Kohlenhydrate finden Sie unter www.sigma-aldrich.com/enzymeexplorer
Substrat
Produkt-Nr.
Beschreibung
Preisangaben
Signalwort
Danger
H-Sätze
Gefahreneinstufungen
Resp. Sens. 1
Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 1
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Persönliche Schutzausrüstung
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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The current production of bioethanol based on lignocellulosic biomass (LCB) highly depends on thermostable enzymes and extremophiles owing to less risk of contamination. Thermophilic bacterial cellulases are preferred over fungi due to their higher growth rate, presence of complex multi-enzymes
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ACS omega, 5(38), 24780-24789 (2020-10-06)
A major challenge in converting lignocellulose to biofuel is overcoming the resistance of the biomass structure. Herein, sequential dilute acid-alkali/aqueous ammonia treatment was evaluated to enhance enzymatic hydrolysis of poplar biomass by removing hemicellulose first and then removing lignin with
Structural organization and a standardized nomenclature for plant endo-1, 4-beta-glucanases (cellulases) of glycosyl hydrolase family 9
Urbanowicz BR, et al.
Plant Physiology, 144(4), 1693-1696 (2007)
E Schuster et al.
Applied microbiology and biotechnology, 59(4-5), 426-435 (2002-08-13)
Aspergillus niger is one of the most important microorganisms used in biotechnology. It has been in use already for many decades to produce extracellular (food) enzymes and citric acid. In fact, citric acid and many A. niger enzymes are considered
Paulina Tomaszewska et al.
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Diploid and polyploid Urochloa (including Brachiaria, Panicum and Megathyrsus species) C4 tropical forage grasses originating from Africa are important for food security and the environment, often being planted in marginal lands worldwide. We aimed to characterize the nature of their
Protokolle
To standardize an enzymatic assay procedure of cellulase.
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Can you please provide the percent purity of the product?
1 answer-
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Does product C1184 have hemicellulase activity, such as xylanase? I am looking for cellulase without hemicellulase activity.
1 answer-
Hemicellulase activity of this product has not been tested.
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What is the solution stability of Cellulase, Product C1184?
1 answer-
Sigma-Aldrich has not determined the solution stability for this product. However, it is reported in Methods in Enzymology, 160, 264 (1988) that cellulase from Aspergillus niger is "completely stable over the range of pH 5.0-8.0 at 4°C for 24 hours and retains about 50% of its original activity after heating at 70°C for 10 minutes. The enzyme is completely inactivated by heating at 80°C for 10 minutes".
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What is the molecular weight of Product C1184, Cellulase from Aspergillus niger?
1 answer-
The molecular weight is 26,000 Daltons.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Are there any additives to Cellulase, Product C1184, in addition to the enzyme itself?
1 answer-
Per information from our supplier, maltodextrin is an extender in this product. Maltodextrin can vary in glucose chain lengths of 2-12. There can also be some free glucose in the product. Either sorbitol or propylene glycol is added to the product as a stabilizer during processing. These components may be "flashed off" during processing, but some small amount may still be present.
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