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A6559

Sigma-Aldrich

Adenosin-5′-triphosphat Dinatriumsalz -Lösung

Crystalline ATP, HPLC purified, aqueous solution for RNA transcription

Synonym(e):

ATP

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25 μMOL
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25 μMOL
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About This Item

CAS-Nummer:
987-65-5
MDL-Nummer:
MFCD00080339
UNSPSC-Code:
41106305
eCl@ss:
32160414
PubChem Substanz-ID:
24891106
NACRES:
NA.52

CHF 205.00

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Qualitätsniveau

300

Form

liquid

Konzentration

100 mM in H2O (adjusted to pH 7 with Trizma®
base)

Fremdaktivität

DNase, RNase, none detected

Versandbedingung

dry ice

Lagertemp.

−20°C

SMILES String

[Na+].[Na+].Nc1ncnc2n(cnc12)[C@@H]3O[C@H](COP(O)(=O)OP([O-])(=O)OP(O)([O-])=O)[C@@H](O)[C@H]3O

InChI

1S/C10H16N5O13P3.2Na/c11-8-5-9(13-2-12-8)15(3-14-5)10-7(17)6(16)4(26-10)1-25-30(21,22)28-31(23,24)27-29(18,19)20;;/h2-4,6-7,10,16-17H,1H2,(H,21,22)(H,23,24)(H2,11,12,13)(H2,18,19,20);;/q;2*+1/p-2/t4-,6-,7-,10-;;/m1../s1

InChIKey

TTWYZDPBDWHJOR-IDIVVRGQSA-L

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Allgemeine Beschreibung

Adenosin-5′-triphosphat-dinatriumsalzlösung eignet sich zur Verwendung in molekularbiologischen Anwendungen wie Kinasereaktionen oder DNA-abhängige RNA-Polymerase-Transkription.

Anwendung

Adenosin-5′-triphosphat-dinatriumsalzlösung wird wie folgt verwendet:
  • Als Standard zum Beurteilen der extrazellulären ATP-Freisetzung aus Randzonenzellen der Stria vascularis[1]
  • Als Komponente der internen Pipettenlösung zum Etablieren einer Aufzeichnungselektrode zur Untersuchung der Neuritenstreckung[2]
  • im In-vitro-VPS34 (katalytische Untereinheit des Phosphatidylinositol-3-Kinase (Ptdlns3K)-Komplexes)-Aktivitätsassay[3]
  • als Komponente von Lyse- und Neuversiegelungspuffern für ATP-Studien[4]
  • bei der spektrofotometrischen Messung des H+-Transports in mit Fluoreszeinisothiocyanat (FITC)-Dextran beladenen A549- (menschliche alveoläre Adenokarzinom-Basalepithelzellen) und HeLa-Zellen[5]
  • zur Einleitung der Pryoptose in mit Lipopolysaccharid behandelten fibroblastenähnlichen Synoviozyten (FLS)[6]

Biochem./physiol. Wirkung

Adenosin-5′-triphosphat (ATP) ist ein wichtiger Energieträger in allen lebenden Organismen. Es besitzt ein hohes Phosphattransferpotenzial. ATP ist an mehreren biologischen Vorgängen wie Membrantransport, Muskelkontraktion und -synthese sowie dem Abbau von biologischen Molekülen beteiligt. Es spielt als intra- und extrazelluläres Signalmolekül eine Rolle in bestimmten zellulären Vorgängen wie der Zellmotilität, der Organentwicklung, der Neurotransmission und der Insulinsekretion.[7]

Rechtliche Hinweise

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Hier finden Sie alle aktuellen Versionen:

Analysenzertifikate (COA)

Lot/Batch Number
0000394151
0000366958
0000394151
0000366958
0000290747

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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Cooper GM
The Cell: A Molecular Approach (2000)
Bin Liu et al.
Cell calcium, 76, 62-71 (2018-10-03)
Adenosine triphosphate (ATP) is stored as lysosomal vesicles in marginal cells of the stria vascular in neonatal rats, but the mechanisms of ATP release are unclear. Primary cultures of marginal cells from 1-day-old Sprague-Dawley rats were established. P2Y2 receptor and
DRAM1 regulates autophagy flux through lysosomes.
Zhang XD
PLoS ONE, 8(5), e63245-e63245 (2013)
James Franklin Tucker et al.
PLoS genetics, 12(2), e1005873-e1005873 (2016-02-20)
Epigenetic gene silencing plays a critical role in regulating gene expression and contributes to organismal development and cell fate acquisition in eukaryotes. In fission yeast, Schizosaccharomyces pombe, heterochromatin-associated gene silencing is known to be mediated by RNA processing pathways including
Yahui Huang et al.
Archiv der Pharmazie (2018-06-29)
Inhibition of spleen tyrosine kinase (Syk) is a promising strategy for the treatment of various allergic and autoimmune disorders such as asthma, rheumatoid arthritis, and allergic rhinitis. Previously, a Syk inhibitor with novel indazole scaffold was discovered by structure-based virtual
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Questions

1–9 of 9 Questions  

  1. How does the storage temperature relate to shipping conditions?

    1 answer

    1. The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.

      Helpful?

  2. Can this preparation of Product A6559, Adenosine 5´-triphosphate disodium salt solution, be used as an ATP standard?

    1 answer

    1. This preparation of ATP is not recommended for use as an ATP standard. The recommended ATP products for use as a standard in bioluminescence experiments is Product Code FL-AAS (preweighed vials of 1 mg each = 2 micromole).

      Helpful?

  3. What is the binding affinity of ATP (Product No. A6559) for Mg++ and other metal ions?

    1 answer

    1. ATP has intrinsic metal binding affinity. The binding constant for various metals are (given as per mole): Mg++, 9554; Na+, 13; Ca++, 3722; K+, 8; Sr++, 1381; Li+, 25.  The ability of ATP to chelate magnesium results in effective Mg++ ion concentrations lower than the total Mg ion concentration.  For example, in a reaction with 1.5mM Mg++ and 0.2 mM ATP, the effective Mg ion concentration is ~1.3mM.

      Helpful?

  4. Can Product A6559, Adenosine 5´-triphosphate disodium salt solution, be used in molecular biology applications?

    1 answer

    1. This is a Molecular Biology grade ATP. It is certified free of DNase and RNase. This product is recommended for any typical molecular biology application.

      Helpful?

  5. Can Product A6559, Adenosine 5´-triphosphate disodium salt solution, be used for in vitro transcription?

    1 answer

    1. The purity of this product should be sufficient for use in T7 RNA polymerase-catalyzed in vitro transcription assay, but we do not test the product for use in that application in our quality control process.

      Helpful?

  6. How long can aqueous ATP solutions like Product No. A6559 be stored?

    1 answer

    1. Aqueous solutions of ATP are stable for months when frozen in solution at -15 °C and for approximately one week at 0 °C.  As with most products, we guarantee the quality of this material for 1 year when stored unopened at the recommended storage temperature (-20°C in this case).

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  7. What is the millimolar extinction coefficient for ATP, Product No. A6559?

    1 answer

    1. The millimolar extinction coefficient (EmM) is 15.4, measured at 259 nm for a sample in 100 mM phosphate buffer, pH 7.0.

      Helpful?

  8. What is meant by 25UMO in the size description of Product A6559, Adenosine 5´-triphosphate disodium salt solution?  What is the product dissolved in?

    1 answer

    1. 25UMO means 25 micromoles.  The 25 UMO size is equivalent to 250 μl of a 100 mM solution. This product is a 100 mM solution in water; the solution pH has been adjusted with Trizma base but contains no additional buffer or salts.

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  9. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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