A3429 and A3179 are the same solution but in different package sizes. No dilution is required for these products, but some customers may prefer a slightly weaker acid differentiation solution. The differentiation time may vary due to staining time and the thickness of the tissue sections being stained.
A3429
Differentiation Solution
for the differentiation of regressive hematoxylin stains
Synonym(e):
Acid Alcohol
Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise
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4 L
CHF 234.00
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4 L
CHF 234.00
About This Item
Empfohlene Produkte
Form
solution
Haltbarkeit
40 mo.
Farbe
colorless
Anwendung(en)
hematology
histology
Lagertemp.
room temp
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Allgemeine Beschreibung
Differentiation solution comprises acidified ethanol or isopropanol that is used to decolorize hematoxylin stain. The duration of exposure to the solution determines the degree of decolorization. Differentiation is a crucial step in hematoxylin-eosin staining to achieve the proper color contrast necessary for visualization, by selectively removing excess hematoxylin from the tissue section and leaving behind the desired staining intensity.[1]
Anwendung
Acidified alcohol solution for the differentiation of regressive hematoxylin stains.
Leistungsmerkmale und Vorteile
- Desired staining patterns can be obtained by varying the degree of exposure to the differentiation solution.
- Results are sharper and more rapid compared to other differentiation methods.[4]
- Crisp and clear differentiation between cellular structures is obtainable when performed according to Sigma-Aldrich Procedure No. GHS.
Prinzip
In regressive hematoxylin staining, a highly concentrated hematoxylin solution is used to rapidly overstain both nuclei and cytoplasm, and the cytoplasm is subsequently decolorized and excess hematoxylin is removed from the nucleus with dilute acid. Improper differentiation leaves excess residual hematoxylin that obscures the fine details of nuclear structures and chromatin and prevents eosin uptake.[1]
Signalwort
Danger
H-Sätze
Gefahreneinstufungen
Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1 - STOT SE 2
Zielorgane
Eyes,Central nervous system
Lagerklassenschlüssel
3 - Flammable liquids
WGK
WGK 1
Flammpunkt (°F)
71.6 °F
Flammpunkt (°C)
22 °C
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Do Differentiation Solutions A3179 and A3429 need to be diluted before use?
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