A3429 and A3179 are the same solution but in different package sizes. No dilution is required for these products, but some customers may prefer a slightly weaker acid differentiation solution. The differentiation time may vary due to staining time and the thickness of the tissue sections being stained.
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A3429
Differentiation Solution
for the differentiation of regressive hematoxylin stains
Synonym(e):
Acid Alcohol
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| Größe/SKU | Verfügbarkeit | Preis |
|---|---|---|
4 L | Warenkorb auf Verfügbarkeit prüfen | CHF 238.00 |
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Unterstützung erhaltenQuality Segment
form
solution
shelf life
40 mo.
color
colorless
application(s)
hematology
histology
storage temp.
room temp
General description
Differentiation solution comprises acidified ethanol or isopropanol that is used to decolorize hematoxylin stain. The duration of exposure to the solution determines the degree of decolorization. Differentiation is a crucial step in hematoxylin-eosin staining to achieve the proper color contrast necessary for visualization, by selectively removing excess hematoxylin from the tissue section and leaving behind the desired staining intensity.
Application
Differentiation solution is employed mainly for the differentiation of regressive hematoxylin stains. It has been used in the following studies:
Acidified alcohol solution for the differentiation of regressive hematoxylin stains.
Biochem/physiol Actions
In regressive hematoxylin staining, a highly concentrated hematoxylin solution is used to rapidly overstain both nuclei and cytoplasm, and the cytoplasm is subsequently decolorized and excess hematoxylin is removed from the nucleus with dilute acid. Improper differentiation leaves excess residual hematoxylin that obscures the fine details of nuclear structures and chromatin and prevents eosin uptake.
Features and Benefits
- Desired staining patterns can be obtained by varying the degree of exposure to the differentiation solution.
- Results are sharper and more rapid compared to other differentiation methods.
- Crisp and clear differentiation between cellular structures is obtainable when performed according to Sigma-Aldrich Procedure No. GHS.
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Dieser Artikel | |||
|---|---|---|---|
| description for the differentiation of regressive hematoxylin stains | description - | description - | description NutriSelect® Plus, powder, for bacteria, pack of 500 g, suitable for microbiology |
| color colorless | color colorless | color - | color - |
| application(s) hematology | application(s) hematology | application(s) - | application(s) bioburden testing |
| form solution | form solution | form - | form powder |
| storage temp. room temp | storage temp. room temp | storage temp. −20°C | storage temp. - |
| shelf life 40 mo. | shelf life 40 mo. | shelf life - | shelf life limited shelf life, expiry date on the label |
| Quality Level 200 | Quality Level 200 | Quality Level - | Quality Level 200 |
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Signalwort
Danger
Gefahrencodes
Sicherheitshinweise
Hazard Classifications
Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1 - STOT SE 2
Zielorgane
Eyes,Central nervous system
Lagerklasse
3 - Flammable liquids
Flammpunkt (°F)
71.6 °F
Flammpunkt (°C)
22 °C
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Do Differentiation Solutions A3179 and A3429 need to be diluted before use?
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