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I0765

Sigma-Aldrich

Immersionsöl

viscosity 150 cSt (lit.)

Synonym(e):

immersion medium for light microscopy

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About This Item

UNSPSC-Code:
12171500
NACRES:
NA.47

Biologische Quelle

synthetic

Qualitätsniveau

200

Farbe

yellow

Viskosität

150 cSt(lit.)

Dichte

0.9 g/cm3 at 23 °C

Anwendung(en)

hematology
histology

Lagertemp.

room temp

Verwandte Kategorien

Allgemeine Beschreibung

Immersion Oil has a defined refractive index of about 1.516. It is important that the refractive index (nD) is about 1.5, similar to that of glass. This enables a homogeneous oil immersion to be achieved. Immersion media are used in conjunction with immersion objectives and are located between the surface of the specimen and the lens of the microscope. Immersion oils practically eliminate light beam deflection so that the effectiveness of the lens is considerably increased.

Anwendung

Immersion oil has been used in the following applications:
  1. in epifluorescent microscopy and cell identification
  2. in the assembly and filling of the microinjecting system
  3. in microscopy to reliably visualize dendritic spines

Biochem./physiol. Wirkung

Immersion oil has a larger impact on 3D resolution. Specifically, matching the immersion oil refractive index to glass, reliably enhances the ability to visualize dendritic spines.

Signalwort

Danger

Gefahreneinstufungen

Aquatic Acute 1 - Aquatic Chronic 1 - Asp. Tox. 1 - Skin Irrit. 2

Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 2

Flammpunkt (°F)

159.8 °F

Flammpunkt (°C)

71 °C

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Analysenzertifikate (COA)

Lot/Batch Number
MKCV4527
MKCS1883
MKCR3290
MKCS2908
MKCQ3730

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Die Dokumentenbibliothek aufrufen

A simple pressure microinjecting system for delivery of small substance volumes to the brain: application to the developmental study of thalamo-cortical projections in foetal and neonatal rats.
Sbriccoli A, et al.
Brain Res. Protoc., 3(3), 257-263 (1999)
Impact of immersion oils and mounting media on the confocal imaging of dendritic spines.
Peterson BM, et al.
Journal of Neuroscience Methods, 242, 106-111 (2015)

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