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Merck

F5415

Sigma-Aldrich

Ficoll® solution

Type 400, 20% in H2O

Synonym(e):

Ready-to-use density gradient media

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About This Item

CAS-Nummer:
MDL-Nummer:
UNSPSC-Code:
12352207
NACRES:
NA.75

Sterilität

0.2 μm filtered

Qualitätsniveau

Typ

Type 400

Form

liquid

Konzentration

20% in H2O

Farbe

clear colorless

Löslichkeit

water: soluble

Dichte

1.055-1.075 g/mL

Anwendung(en)

hematology
histology

Lagertemp.

2-8°C

Allgemeine Beschreibung

Ficoll® Solution is a highly branched polymer of sucrose, also known as polydisperse mixtures of dextran. Ficoll® molecules are inert and not re-absorbed by proximal tubules. The structure is open and deformable, and not a rigid sphere thereby becoming a preferred choice in “crowding” experiments.
Used in electrophoresis and as a hapten carrier. Most commonly used to prepare density gradients.

Anwendung

Ficoll® has been used in


  • Isolation of cord blood mononuclear cells
  • Isolation of CD133 negative cells
  • Isolation and expansion of circulating endothelial progenitor cells.

Sonstige Hinweise

A nonionic synthetic polymer of sucrose.

Rechtliche Hinweise

Ficoll is a registered trademark of Cytiva

Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


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Analysenzertifikate (COA)

Lot/Batch Number

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Die Dokumentenbibliothek aufrufen

Circulation enrichment of functional endothelial progenitor cells by infantile phototherapy.
Siavashi V, et al.
Journal of Cellular Biochemistry, 118(2), 330-340 (2017)
Juliane Schmidt et al.
PloS one, 6(3), e18184-e18184 (2011-03-30)
The characterization of factors contributing to the formation and development of surface-associated bacterial communities known as biofilms has become an area of intense interest since biofilms have a major impact on human health, the environment and industry. Various studies have
Junchao Xing et al.
Tissue engineering. Part C, Methods, 25(3), 137-147 (2019-02-09)
Patient-specific individual tissue-engineered bones (iTEBs) have been recognized as a promising strategy for treating large bone defects. However, current construction protocols of iTEBs vary between lots and lack standardization and quality control, hampering further research and application. This study was
Effect of mononuclear cells on morphometric of kidney parameters in hyperglycemic rat.
Salmani F, et al.
Annals of Experimental Biology, 2(3), 1-7 (2014)
Analysis of Structural and Functional Glomerular Changes in Hyperglycemic Rats that Treated with Human Umbilical Cord Blood Derived CD133 Negative Ccells.
Feizi F, et al.
Physical Chemistry, 1(1) (2014)

Protokolle

Hematopoietic stem cell culture protocols for isolation, expansion, and differentiation of CD34+ cells including CFU assays.

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