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B4380

Sigma-Aldrich

Bromobiman

≥97% purity, powder

Synonym(e):

Monobrombiman

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About This Item

Empirische Formel (Hill-System):
C10H11BrN2O2
CAS-Nummer:
71418-44-5
Molekulargewicht:
271.11
Beilstein:
4430959
MDL-Nummer:
MFCD00036951
UNSPSC-Code:
12171500
PubChem Substanz-ID:
24891769
NACRES:
NA.47

product name

Bromobiman, ≥97% (HPLC)

Qualitätsniveau

200

Assay

≥97% (HPLC)

Form

powder

Farbe

yellow

mp (Schmelzpunkt)

161 °C

Löslichkeit

acetonitrile: 20 mg/mL

ε (Extinktionskoeffizient)

4.6-5.1 at 396-398 nm in H2O

Anwendung(en)

diagnostic assay manufacturing
hematology
histology

Lagertemp.

−20°C

SMILES String

CC1=C(C)C(=O)N2N1C(CBr)=C(C)C2=O

InChI

1S/C10H11BrN2O2/c1-5-7(3)12-8(4-11)6(2)10(15)13(12)9(5)14/h4H2,1-3H3

InChIKey

AHEWZZJEDQVLOP-UHFFFAOYSA-N

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Allgemeine Beschreibung

Bromobimane is also known as monobromobimane. It is also a known probe for thiols and is a fluorescent reagent activated upon a photolysis reaction.

Anwendung

Fluoreszenzfarbstoff für Thiole

Biochem./physiol. Wirkung

Bromobimane in solution reacts with small thiol groups (e.g., GSH) and with reactive protein thiol groups (e.g., hemoglobin). The reaction of Bromobimane with thiols is of second-order and dependent on pH and upon reacting with thiolate, it activates the water-soluble fluorescent product for detection.

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)

Choose from one of the most recent versions:

Analysenzertifikate (COA)

Lot/Batch Number
0000385726
0000369257
0000369257
0000385726
SLCC7229

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A method based on the differential reactivity of thiol and thiolate with monobromobimane (mBBr) has been developed to measure nucleophilicity and acidity of protein and low-molecular-weight thiols. Nucleophilicity of the thiolate is measured as the pH-independent second-order rate constant of
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