11699121001

PCR Buffer Set

suitable for PCR, pkg of 2 × 2 mL

• UNSPSC-Code: 41116133

Eigenschaften

• Verpackung: pkg of 2 × 2 mL
• Hersteller/Markenname: Roche
• Methode(n): PCR: suitable
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

The kit-provided PCR buffer without MgCl₂, in combination with the kit-provided MgCl₂ stock solution, are used for the individual adjustment of the Mg²⁺ concentration. In most PCR applications, a concentration of 1.5 mM MgCl₂ will yield satisfactory results with a dNTP concentration of 200 μM for each nucleotide. To optimize the PCR, titrate to determine the optimal Mg²⁺ concentration for high PCR specificity and amplicon yield.

Anwendung

For optimization of the magnesium-chloride concentration in the polymerase chain reaction (PCR) using Taq DNA Polymerase.

Verpackung

1 set containing 2 components

1 set containing 3 components

Qualität

Function test: Each lot of buffer is tested in PCR.
Absence of contaminants: No exo- or endonuclease activity is detectable according to the current Quality Control procedures.

Sonstige Hinweise

For life science research only. Not for use in diagnostic procedures.

Nur Kit-Komponenten

• MgCl₂ Stock Solution 25 mM
• PCR Buffer without Mg²⁺ 10x concentrated
• Alkaline Phosphatase Substrate, CSPD
• Enhancer, Emerald II
• Assay Buffer

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): does not flash
• Flammpunkt (°C): does not flash