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11207741910

Roche

Anti-Digoxigenin-Fluorescein, Fab fragments

from sheep

Synonym(e):

anti-digoxigenin, digoxigenin

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About This Item

UNSPSC-Code:
12352203

Biologische Quelle

sheep

Qualitätsniveau

100

Konjugat

fluorescein conjugate

Antikörperform

purified immunoglobulin

Antikörper-Produkttyp

primary antibodies

Klon

polyclonal

Form

lyophilized

Verpackung

pkg of 200 μg

Hersteller/Markenname

Roche

Isotyp

IgG

Lagertemp.

2-8°C

Verwandte Kategorien

Allgemeine Beschreibung

Anti-Digoxigenin-Fluorescein, Fab fragments has fab fragments from polyclonal anti-digoxigenin antibodies, conjugated to fluorescein.

Spezifität

The polyclonal antibody from sheep is specific to digoxigenin and digoxin and shows no cross-reactivity with other steroids, such as human estrogens and androgens.

Anwendung

Anti-Digoxigenin-Fluorescein, Fab fragments are used for the detection of digoxigenin-labeled compounds using:
  • Digoxigenin-labeled sugars in glycoconjugate research
  • Fluorescent in situ hybridization (FISH)
  • Immunohistocytochemistry
  • In situ hybridization
Anti-Digoxigenin-Fluorescein, Fab fragments cannot be used for membrane applications.

Angaben zur Herstellung

  • Working concentration: Working concentration of conjugate depends on application and substrate. The following concentrations should be taken as a guideline:Detection of digoxigenin-labeled sugars in glycoproteins: 50 to 20μg/ml
  • Fluorescent in situ hybridization (FISH): 1 to 20μg/ml
  • Immunohistocytochemistry: 20 to 50μg/ml
  • In situ hybridization: 20 to 50μg/ml

The anti-DIG anitbody is highly specific and sensitive. Therefore, for FISH, a concentration of 1μg/ml is sufficient, and only in case of critical probes, this concentration may be raised to <10μg/ml (with a distinct background to be expected).
Working solution: Reconstitution
Add 1 ml double-distilled water to a final concentration of 200μg/ml.

Blocking:
PBS, 0.5% bovine serum albumin (w/v), pH 7.4.
1% Blocking reagent (w/v), 1 to 5% heat inactivated fetal calf serum (v/v) or sheep normal serum can be used for reduction of unspecific binding. Furthermore, pH can be increased up to pH 8.5 to 9.0.
Storage conditions (working solution): Always prepare fresh!
After immunization with digoxigenin, sheep IgG was purified by ion-exchange chromatography, and the specific IgG was isolated by immunosorption. The Fab fragments obtained by papain digestion were purified by gel filtration, conjugated to the specific label, and stabilized in buffer.

Rekonstituierung

Add 1 ml double-distilled water to a final concentration of 200 μg/ml.

Sonstige Hinweise

For life science research only. Not for use in diagnostic procedures.

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Piktogramme

Exclamation mark
GHS07

Signalwort

Warning

H-Sätze

H317,H412

Gefahreneinstufungen

Aquatic Chronic 3 - Skin Sens. 1

Lagerklassenschlüssel

13 - Non Combustible Solids

WGK

WGK 2

Flammpunkt (°F)

does not flash

Flammpunkt (°C)

does not flash

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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