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SCM075

Sigma-Aldrich

ROCK-Inhibitor (Y-27632)

The ROCK Inhibitor (Y-27632) is available in a 5 mg format & has been optimized & validated for cell culture & Neuroscience applications.

Synonym(e):

Y-27632

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About This Item

UNSPSC-Code:
12352207
eCl@ss:
32160801
NACRES:
NA.75

Qualitätsniveau

100

Form

liquid

Mol-Gew.

320.26 g/mol

Methode(n)

cell culture | mammalian: suitable

Versandbedingung

dry ice

Verwandte Kategorien

Allgemeine Beschreibung

Der ROCK-Inhibitor (Y-27632) ist ein selektiver, ATP-kompetitiver Inhibitor der Rho-assoziierten Proteinkinase (ROCK), einschließlich p160Rock (Ki=140nM) und Rock-II. Agiert auch als potenter Inhibitor der agonist-induzierten Ca2+-Sensibilisierung der Myosinphosphorylierung und Kontraktion der glatten Muskulatur. Eine einstündige Behandlung mit 10uM Y-27632 blockiert die Apoptose dissoziierter kultivierter menschlicher embryonaler Stammzellen (hESCs) und erhöht das Überleben und die Kloneffizienz von hESCs, ohne ihre Pluripotenz zu beeinträchtigen1. Vor kurzem ging aus einigen Studien hervor, dass die Anwendung von Y-27632 und Accutase (Kat. Nr. SCR005), einer formulierten Mischung von Verdauungsenzymen, die Überlebensrate einzelner hESCs nach dem Auftauen erhöht, wenn konventionelle Methoden des langsamen Einfrierens und schnellen Auftauens angewendet werden.

Lagerung und Haltbarkeit

Vor der Rekonstitution: Lichtgeschützt bei -20 ºC 2 Jahre lang haltbar. Nach der Rekonstitution: Lichtgeschützt bei -20 ºC 1 Jahr lang

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

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Maike Frye et al.
eLife, 9 (2020-09-09)
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Ambra Gianotti et al.
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Marta Słoniecka et al.
Journal of molecular medicine (Berlin, Germany), 97(10), 1477-1489 (2019-08-11)
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Anushree C Gulvady et al.
Molecular biology of the cell, 30(11), 1298-1313 (2019-03-21)
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