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Merck
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Key Documents

R2283

Sigma-Aldrich

Pepsin Reagent, Ready to Use, Antigen Retriever

suitable for immunohistochemistry

Synonyme(s) :

pepsin

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About This Item

Code UNSPSC :
12171500
Nomenclature NACRES :
NA.47

Source biologique

Porcine stomach

Niveau de qualité

Application(s)

hematology
histology

Température de stockage

2-8°C

Application

Pepsin is used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies in immunohistochemistry (IHC).
Pepsin is used for proteolytic digestion of formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies. In immunohistochemistry (IHC), most commonly used fixatives such as formalin mask tissue antigens (cellular, membrane, and nuclear) by their intrinsic crosslinking. This masking results in poor or no staining in IHC. Pepsin digestion of FFPE tissue sections improves accessibility of antibodies to tissue antigens. Ready to use pepsin reagent, antigen retriever has been used for antigen unmasking in immunohistochemistry.

Pictogrammes

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Mention d'avertissement

Warning

Mentions de danger

Classification des risques

Skin Sens. 1

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


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Fetal testis organ culture reproduces the dynamics of epigenetic reprogramming in rat gonocytes.
Rwigemera A, et al.
Epigenetics & Chromatin, 10(1), 19-19 (2017)
Codistribution of collagen type IV and laminin in liver fibrosis of elderly cadavers: immunohistochemical marker of perisinusoidal basement membrane formation.
Mak Ki M, et al.
The Anatomical Record, 296(6)A, 953-964 (2013)
The effect of low doses of cadmium and zinc chloride on the Blood-Testis Barrier of Sprague Dawley rats.
Redondo A, et al.
arXiv (2012)
M Bekhouche et al.
Journal of materials chemistry. B, 8(36), 8422-8432 (2020-08-18)
Hydrogel-based regenerative endodontic procedures (REPs) are considered to be very promising therapeutic strategies to reconstruct the dental pulp (DP) tissue in devitalized human teeth. However, the success of the regeneration process is limited by residual bacteria that may persist in
Elizabeth Vinod et al.
Acta histochemica, 122(1), 151462-151462 (2019-11-18)
Interest in chondroprogenitors arose due to their inherent stem cell like properties, and their initial characterization was based on identification of a small percentage of CD49e positive cells in cultured chondrocytes (CC). It was further noted that when fresh chondrocytes

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