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10108014001

Roche

Papain

from Carica papaya

Synonyme(s) :

cystein protease, papain

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About This Item

Numéro de classification (Commission des enzymes):
3.4.22.2 (BRENDA, IUBMB)
Code UNSPSC :
12352204

Source biologique

papaya

Niveau de qualité

100

Forme

suspension

Activité spécifique

30 U/mg
~30 units/mg protein (At 25 °C with BAEE as the substrate.)

Poids mol.

23 kDa

Conditionnement

pkg of 10 mL (100 mg)

Fabricant/nom de marque

Roche

Concentration

10-100 mg/mL

pH optimal

6.0-7.0

Conditions d'expédition

wet ice

Description générale

Papain is a cysteine protease of the papain family. It is a single chain protein. The molecular weight of papain is 23 kDa. Papain is composed of two structural domains and a cleft between them, which consists of the active site.

Application

Papain has been used in the digestion of various brain cells.
Use Papain for the complete proteolytic cleavage of proteins, limited hydrolysis of native immunoglobulins, and tissue dissociation (together with collagenase, esterase, and trypsin). The enzyme solubilizes integral membrane proteins and produces glycopeptides from purified proteoglycans. The addition of cysteine (approximately 0.5% [w/v]) is essential for enzyme activity.

Actions biochimiques/physiologiques

Papain exhibits proteolytic activity against amide links, amino acid esters, peptides, proteins. It mainly cleaves peptide bonds containing amino acids such as lysine, arginine and residues succeeding phenylalanine. It can catalyze several reactions, such as hydrolysis, transferase action, specificity and acyl-enzyme intermediate.

Caractéristiques et avantages

Inhibitors: SH-blocking reagents, iodoacetic acid, iodoacetamide, TPCK, TLCK, leupeptin, a2-macroglobulin, E-64, PMSF, and antipain, Hg2+, and other heavy metals

Forme physique

Suspension, crystalline, nonsterile

Notes préparatoires

Working concentration: 0.05 to 0.5 mg/ml

Stockage et stabilité

Store at 2 to 8 °C. (a decrease in activity of 20% may occur within 6 months)

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

nwg

Point d'éclair (°F)

does not flash

Point d'éclair (°C)

does not flash

Certificats d'analyse (COA)

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John S. White and Dorothy C. White
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Wang W and Townes-Anderson E
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The structure of papain
Advances in Protein Chemistry, 25, 79-115 (1971)
Logan A Walker et al.
Journal of translational medicine, 18(1), 63-63 (2020-02-11)
Direct cDNA preamplification protocols developed for single-cell RNA-seq have enabled transcriptome profiling of precious clinical samples and rare cell populations without the need for sample pooling or RNA extraction. We term the use of single-cell chemistries for sequencing low numbers
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