This falls under the research grade.
P7125
Pepsine from porcine gastric mucosa
powder, ≥400 units/mg protein
Synonyme(s) :
Pepsine from hog stomach, Pepsine A
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About This Item
Le tarif et la disponibilité ne sont pas disponibles actuellement.
Produits recommandés
Source biologique
Porcine gastric mucosa
Niveau de qualité
Forme
powder
Activité spécifique
≥400 units/mg protein
Poids mol.
35 kDa
Solubilité
10 mM HCl: soluble 1.0 mg/mL, clear to faintly turbid, colorless
Numéro d'accès UniProt
Température de stockage
2-8°C
Informations sur le gène
pig ... LOC396892(396892)
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Catégories apparentées
Application
La pepsine peut être utilisée pour produire des fragments F(ab′)2 d′anticorps. pepsine sur www.sigma-aldrich.com/enzymeexplorer.
Pepsin is a peptidase used to digest proteins and is commonly used in the preparation of Fab fragments from antibodies. Pepsin, from porcine gastric mucosa, has been used to hydrolyze dry cervical samples in mice. Product P7125 is provided in powder form. Product P7125 has been used to denature DNA from kidney cells[1] and to digest pathology samples from anal canal carcinomas (ACC) biopsies prior to EGFR staining[2].
Actions biochimiques/physiologiques
Clivage préférentiel : résidus hydrophobes et aromatiques en positions P1 et P1′. Clive les liaisons Phe-Val, Gln-His, Glu-Ala, Ala-Leu, Leu-Tyr, Tyr-Leu, Gly-Phe, Phe-Phe et Phe-Tyr dans la chaîne β d′insuline.
It does not cleave at valine, alanine, or glycine linkages.[5] Z-L-tyrosyl-L-phenylalanine, Z-L-glutamyl-L-tyrosine, or Z-L-methionyl-L-tyrosine may be used as substrates for pepsin digestion.[5][6] Pepsin is inhibited by several phenylalanine-containing peptides.[7]
Pepsin hydrolyzes peptide bonds, not amide or ester linkages. Pepsin cleaves peptides with an aromatic acid on either side of the peptide bond. Sulfur-containing amino acids increase susceptibility to hydrolysis when they are close to the peptide bond. Pepsin preferentially cleaves at the carboxyl side of phenylalanine and leucine and at the carboxyl side of glutamic acid residues. Cleaves Phe-Val, Gln-His, Glu-Ala, Ala-Leu, Leu-Tyr, Tyr-Leu, Gly-Phe, Phe-Phe and Phe-Tyr bonds in the β chain of insulin
Pepsin is the major proteolytic enzyme produced in the stomach. It digests proteins through the cleavage of interior peptide linkages.[8]
Pepsin is the major proteolytic enzyme produced in the stomach. It digests proteins through the cleavage of interior peptide linkages.[8]
Définition de l'unité
One unit will produce a ΔA280 of 0.001 per min at pH 2.0 at 37 °C, measured as TCA-soluble products using hemoglobin as substrate. (Final volume = 16 mL. Light path = 1 cm.)
Remarque sur l'analyse
Le pH optimal se situe entre 2 et 4. Activité maintenue dans une solution d′urée à 4 M et de guanidine HCl à 3 M. Stable à 60 °C. La pepsine est inactivée de façon irréversible à pH 8,0 - 8,5.
Protein determined by E1%/280
Autres remarques
View more information on pepsin at www.sigma-aldrich.com/enzymeexplorer.
Inhibiteur
Réf. du produit
Description
Tarif
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Organes cibles
Respiratory system
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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Les clients ont également consulté
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Memorias do Instituto Oswaldo Cruz, 102(1), 49-52 (2007-02-13)
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J Dahle et al.
Proceedings of the National Academy of Sciences of the United States of America, 94(5), 1773-1778 (1997-03-04)
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American journal of clinical pathology, 124(1), 20-23 (2005-06-01)
Most anal canal carcinomas (ACCs) are squamous cell carcinomas (SCCs). SCCs in other tumor sites strongly express epidermal growth factor receptors (EGFRs), the inhibition of which might result in favorable changes in tumor growth. A review of the published scientific
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Sweeney, P.J., and Walker, J.M.
Methods in Molecular Biology, 290-291 (1993)
Protocoles
This procedure may be used for determination of Pepsin activity using hemoglobin as the substrate. It is a spectrophotometric stop rate determination.
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Can you please tell me the Grade of this product?
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Hi, What is the shelf life for this product? Thanks
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This product is not assigned an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.
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Which Pepsin do you recommend for histology/antigen retrevial? According to protocol 0.2mg/ml in 0.2M HCL
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This product has not been specifically qualified for antigen retrieval, however, it may be able to be qualified for use by the end user. A typical protocol using pepsin for antigen retrieval suggests the following stock and working solutions:
Pepsin Stock Solution (1% in 10mM HCl):
Pepsin ------------------------------------- 100 mg
10mM HCl (pH 2.0) ---------------------- 10 mlMix to dissolve. Store at -20 ºC
Pepsin Working Solution (0.5% in 5mM HCl):
Pepsin Stock Solution (0.5%) ------------ 1 ml
Distilled water ---------------------------- 1 mlMix well.
For antigen retrieval - cover sections with pepsin working solution and incubate for 10-20 minutes at 37 °C in humidified chamber (optimal incubation time may vary depending on tissue type and degree of fixation, and should be determined by user).
Product R2283 is a pepsin product qualified for antigen retrieval https://www.sigmaaldrich.com/product/sigma/r2283.
For more information, please see the following link: https://www.sigmaaldrich.com/technical-documents/protocol/protein-biology/immunohistochemistry/immunohistochemistry-protocol#antigen_retrieval.
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