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GC Analysis of Terpenes in Hops (Cascade Variety) on Equity-1 after Headspace SPME using 50/30 μm DVB/CAR/PDMS Fiber

GC Analysis of Terpenes in Hops (Cascade Variety) on Equity-1 after Headspace SPME using 50/30 μm DVB/CAR/PDMS Fiber suitable for GC, application for SPME

Materiais

Fibra para SPME

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Conjunto de fibras para SPME de divinilbenzeno-carboxeno-polidimetilsiloxano (DVB/CAR/PDMS)

needle size 23 ga, StableFlex, for use with autosampler

acessório

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Inlet Liner, Direct (SPME) Type, Straight Design (unpacked)

pkg of 1 ea

Headspace vial, screw top, rounded bottom (vial only)

volume 10 mL, clear glass vial, thread for 18, O.D. × H 22.5 mm × 46 mm, pkg of 100 ea

Tampa de rosca magnética para frascos de headspace, rosca de 18 mm

PTFE/silicone septum (white PTFE/blue silicone), septum thickness 1.5 mm, pkg of 100 ea

Molded Thermogreen® LB-2 Septa, with injection hole

diam. 11 mm, pkg of 50 ea

coluna analítica

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Equity-1 Capillary GC Column

L × I.D. 60 m × 0.25 mm, df 0.25 μm

padrão

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C7 - C40 Saturated Alkanes Standard

certified reference material, 1000 μg/mL each component in hexane

CONDITIONS

sample/matrix

ground Cascade variety hop pellets

SPME fiber

Divinylbenzene/Carboxen/Polydimethylsiloxane (DVB/CAR/PDMS), 50/30 μm (57298-U)

extraction

20 min, headspace, 40 °C

desorption process

3 min, 270 °C

sample preparation

30 min equilibration at 40 °C prior to extraction

sample preparation

fiber postbake after extraction, 3 min at 270 °C

column

Equity-1, 60 m x 0.25 mm I.D., 0.25 μm (28047-U)

oven

60 °C (2 min), 5 °C/min to 275 °C (5 min)

inj. temp.

270 °C

detector

MSD

MSD interface

300 °C

scan range

full scan, m/z 50-500

carrier gas

helium, 1 mL/min constant flow

liner

0.75 mm ID, SPME

Descrição

Nota de análise

Terpenes are contained in the essential oils derived from plants, and often impart characteristic aromas to the plant and/or oil. In this application, headspace-SPME combined with GC/MS was used to analyze some of the terpenes present in the Cascade variety of hops. Chromatographic separation was performed on an Equity-1 capillary GC column, and identification was done using retention indices and spectral library match. Analysis showed a characteristic terpene profile, with high levels of ß-myrcene, caryophyllene, and humulene present.

Outras notas

add 0.5 gm ground hops to a 10 mL headspace vial

Informações legais

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