LC/MS Analysis of Pilocarpine and Betaxolol in the Presence of Benzalkonium Chlorides on Ascentis^® Express HILIC

column

Ascentis Express HILIC, 15 cm x 4.6 mm I.D., 2.7 μm particles (53981-U)

mobile phase

[A] 2 mM arrmonium acetate, pH 6.7 in [B] 10:90 (v/v) water:acetonitrile

flow rate

1.0 mL/min

pressure

1160 psi (80 bar)

column temp.

35 °C

detector

ESI(+), XlC, m/z 304, 332 (BACs), m/z 209 (pilocarpine), m/z 308 (betaxolol)

injection

2 μL

sample

10 μg/mL each in acetonitrile