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V900483

Sigma-Aldrich

Trizma® base

≥99%, crystalline powder, reagent grade, Vetec™

Sinônimo(s):

2-amino-2-(hidroximetil)-1,3-propanodiol, THAM, Tris base, Tris(hidroximetil)-aminometano, Trometamol

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About This Item

Fórmula linear:
NH2C(CH2OH)3
Número CAS:
77-86-1
Peso molecular:
121.14
Beilstein:
741883
Número CE:
201-064-4
Número MDL:
MFCD00004679
Código UNSPSC:
12161700
ID de substância PubChem:
329829884
Preço e disponibilidade não estão disponíveis no momento.

Nome do produto

Trizma® base, Vetec™, reagent grade, ≥99%

grau

reagent grade

descrição

aminopeptidase substrate

linha de produto

Vetec™

Ensaio

≥99%

Formulário

crystalline powder

pH

10.5-12

faixa de pH útil

7-9

pKa (25 °C)

8.1

p.e.

219-220 °C/10 mmHg (lit.)

pf

167-172 °C (lit.)

solubilidade

water: 0.667 g/mL, clear, colorless to faintly yellow

cadeia de caracteres SMILES

NC(CO)(CO)CO

InChI

1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2

chave InChI

LENZDBCJOHFCAS-UHFFFAOYSA-N

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Descrição geral

Trizma® base is a basimetric standard used in biochemistry and molecular biology. It may be used as a buffer or as a component of mixed buffer formulations. It is essentially stable, relatively non-hygroscopic and has a high equivalent weight.[1][2]

Aplicação

Trizma® base can be used as a buffer:
  • In the evanescent absorption analysis of NADPH.[3]
  • In the autoxidation studies of oxymyoglobin.[4]

Outras notas

Os valores de pH de todos os tampões são dependentes da temperatura e da concentração. Para tampões Tris, o pH aumenta cerca de 0,03 unidade por °C de diminuição na temperatura, e diminui 0,03-0,05 unidades por diluição de 10 vezes.
Para aplicações precisas, use um medidor de pH calibrado com eletrodo de combinação de vidro/calomelano.

Informações legais

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany
Vetec is a trademark of Merck KGaA, Darmstadt, Germany

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

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Certificados de análise (COA)

Lot/Batch Number
WXBF5258V
WXBF2513V
WXBF4131V
WXBF2802V
WXBF2803V

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Autoxidation of native oxymyoglobin. Kinetic analysis of the pH profile
Shikama K and Sugawara Y
European Journal of Biochemistry, 91(2), 407-413 (1978)
Tapered fibers for cell studies
Reviews in Fluorescence (2005)
Preparation of buffers for use in enzyme studies
Handbook of Biochemistry and Molecular Biology (2010)
Tri (hydroxymethyl) aminomethane as Acidimetric Standard.
Fossum J, et al.
Analytical Chemistry, 23(3), 491-493 (1951)
Corey A Baron et al.
Magnetic resonance in medicine, 73(3), 1075-1084 (2014-04-12)
An acquisition method that does not increase scan time or specific absorption rate is investigated for reducing the deleterious effects of cerebrospinal fluid (CSF) partial volume effects on diffusion tensor imaging (DTI) tractography. It is based on using a shorter
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Questions

1–2 of 2 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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